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Composition for treating or preventing inflammatory skin disease, comprising, as active ingredient, immature citrus fruit extract, or synephrine or salt thereof

A technology for skin diseases, active ingredients, applied in the field of compositions for treating or preventing inflammatory skin diseases containing citrus immature fruit extract or synephrine or its salt as active ingredients, able to solve the citrus price collapse, etc. question

Active Publication Date: 2016-02-24
KOREA BIOSITE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, due to environmental changes such as frequent rainfall, humidity, and temperature recently, the citrus produced is immature fruit and citrus with reduced quality, and the mass production of citrus cultivated in greenhouses and other facilities and the preference for imported goods have led to the price of citrus. plummeted

Method used

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  • Composition for treating or preventing inflammatory skin disease, comprising, as active ingredient, immature citrus fruit extract, or synephrine or salt thereof
  • Composition for treating or preventing inflammatory skin disease, comprising, as active ingredient, immature citrus fruit extract, or synephrine or salt thereof
  • Composition for treating or preventing inflammatory skin disease, comprising, as active ingredient, immature citrus fruit extract, or synephrine or salt thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1: the preparation of citrus immature fruit extract

[0064] Unripe citrus was washed and pulverized, and extracted by immersing in ethanol for 5 days. After filtering and concentrating, freeze-drying is carried out, thereby preparing the powder of the citrus immature fruit extract.

Embodiment 2

[0065] Example 2: Isolation and analysis of synephrine from immature citrus fruits

[0066] The powder of the immature citrus fruit extract obtained in the above-mentioned Example 1 was purified by BulkC18 chromatography, and was divided into 11 fractional extracts, which were then passed through a C18 column using pre-high performance liquid chromatography (Prep-HPLC). Chromatography is used to separate the novel active ingredient substance from a second of these fractions. For the structure of the active ingredient substance, instruments such as nuclear magnetic resonance (NMR), infrared radiation (IR), ultraviolet light (UV) are used to confirm that the active ingredient substance is (4-[1-hydroxyl-2-(formazan amino)ethyl]phenol (molecular weight: 167.21), and the general chemical name is synephrine.

Embodiment 3

[0067] Example 3: Determination of the endocrine effect of citrus immature fruit extract or synephrine isolated therefrom on inhibiting eotaxin-1

[0068] Using liposome (superfect) (superfect, Quigen company) to insert eotaxin-1 reporter gene into human normal fibroblasts (humannormalfibroblast, NIH / 3T3, Department of Dermatology, Asia University) and carry out After conversion, at a concentration of 5% CO 2 Cultivate at 37°C for 24 hours in an incubator. Then, the transformed fibroblasts were seeded in 24-well microplates filled with DMEM medium so that each well had about 2×10 5 cells, and treated with 1ppm, 10ppm and 100ppm of immature citrus extract or 10μM, 100μM and 200μM of synephrine, respectively, with 50ng / ml of eotaxin-1 known to induce eotaxin-1 Interleukin-4 (R&D, Minneapolis, MN, IL-4) was co-treated and cells were harvested after 24 hours of incubation. Then, the collected cells were disrupted, reacted with a luciferase substrate to measure luminescence, and...

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Abstract

The present invention relates to a composition for treating or preventing an inflammatory skin disease, comprising, as active ingredients, an immature citrus fruit extract and / or synephrine or salt thereof. The composition of the present invention prevents an inflammatory reaction by inhibiting activity of STAT6, expression of eotaxin-1 and an agglomeration function of eosinophil, and thus, shows effects of preventing and treating an allergic inflammatory disease, atopic dermatitis, eczema, psoriasis, etc. Also, the composition of the present invention does not have cytotoxicity and a skin side effect, and thus, can be safely applied to pharmaceutical products and cosmetics.

Description

technical field [0001] The present invention is a research result completed by the Korean Ministry of Health and Welfare for the research project (Problem No. A103017) on the theme of improving inflammatory skin diseases in the global cosmetics research and development business. Specifically, it relates to a composition for treating or preventing inflammatory skin diseases comprising an extract of unripe citrus fruit, or synephrine or a salt thereof as an active ingredient. Background technique [0002] Atopic dermatitis (Atopic dermatitis) is a chronic inflammatory skin disease characterized by persistent itching and acute and chronic eczema lesions of the skin. In addition to skin lesions, it is also accompanied by asthma and allergic rhinitis. The causes of atopic dermatitis are generally genetic factors, immunological factors and environmental factors. In addition, recently, skin barrier abnormalities are also considered to be the main cause. In addition, it has been r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61K31/085A61P17/00A61P29/00
CPCA61K31/137A61K36/752A61P17/00A61P17/02A61P29/00A61K31/085A61K9/06A61K9/148A61K9/20A61K9/48
Inventor 卢京佰金莹洙郑恩先朴德勋
Owner KOREA BIOSITE CO LTD
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