Method for improving dried orange peel flavonoid content by using aspergillus flavus

A technology of Aspergillus flavus and dried tangerine peel, which is applied in the field of increasing the content of tangerine peel flavonoids, can solve the problems of high cost, long time required, etc., and achieves the effects of strong antioxidant activity, promotion and improvement, and good application prospects.

Inactive Publication Date: 2016-03-09
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the significance of improving the flavonoid content of tangerine peel is significant, yet at present all is to improve the flavonoid content in tangerine peel by

Method used

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  • Method for improving dried orange peel flavonoid content by using aspergillus flavus
  • Method for improving dried orange peel flavonoid content by using aspergillus flavus
  • Method for improving dried orange peel flavonoid content by using aspergillus flavus

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1 The method of the present invention for increasing the content of tangerine peel flavonoids

[0024] 1. Samples and materials

[0025] 1 sample information

[0026] The sample information is shown in Table 1. The sample used in the experiment was identified by Professor Yan Zhuyun of Chengdu University of Traditional Chinese Medicine, and it was the dried and mature peel of the Rutaceae plant Citrusreticulata Blanco and its cultivated varieties.

[0027] Table 1 Experimental sample information

[0028] Sample serial number

[0029] Note: "-" means that the collection time is not clear; "a" means that the sample is used for the reverse connection experiment of fungal strains.

[0030] 2 Instruments and reagents

[0031] RetschMM400 ball mill (Letch, Germany); PTC200PCR instrument (BIO-Rad, US); GelDoxXR gel imaging system (BIO-Rad, US); DYY-8C electrophoresis (Beijing Liuyi Instrument Factory); BI3730XL sequencer ( AppliedBio-systems, USA); KRQ-300P artificial climat...

Example Embodiment

[0077] Example 2 The method of the present invention for increasing the content of tangerine peel flavonoids (Aspergillus flavus)

[0078] 1 samples and materials

[0079] 1.1 Chenpi

[0080] Scarlet Citrusreticulata

[0081] CCPR2 gown a Dahongpao’ Pujiang, Sichuan 2014.11

[0082] The sample was identified by Professor Yan Zhuyun of Chengdu University of Traditional Chinese Medicine, and it is the dried and mature peel of the Rutaceae plant Citrusreticulata Blanco and its cultivated varieties.

[0083] 1.2 Strains

[0084] Aspergillus flavus CGMCC No. 3.6422 and Aspergillus flavus CGMCC No. 3.6151 are all from CGMCC (China Common Microbial Culture Collection and Management Center).

[0085] 2. Treatment method

[0086] Sterilization: Take 15 grams of dried tangerine peel sample and spread it on the bottom of the petri dish, and use ultraviolet light (the intensity of the ultraviolet light is 90μW / CM 2 ; Irradiation distance is 25~30cm) Sterilize for 30min, turn over and continue irradiati...

Example Embodiment

[0100] Example 3 The method of the present invention for increasing the content of tangerine peel flavonoids (Aspergillus flavus)

[0101] 1 samples and materials

[0102] 1.1 Chenpi

[0103] Scarlet Citrusreticulata

[0104] CCPR2 gown a Dahongpao’ Pujiang, Sichuan 2014.11

[0105] The sample was identified by Professor Yan Zhuyun of Chengdu University of Traditional Chinese Medicine as the dried and mature peel of the Rutaceae plant Citrusreticulata Blanco and its cultivated varieties.

[0106] 1.2 Strains

[0107] Aspergillus flavus CGMCC No. 3.6422 and Aspergillus flavus CGMCC No. 3.6151 are all from CGMCC (China Common Microbial Culture Collection and Management Center).

[0108] 2. Treatment method

[0109] Sterilization: Take 15 grams of dried tangerine peel sample and spread it on the bottom of the petri dish, and use ultraviolet light (the intensity of the ultraviolet light is 70μW / CM 2 ; Irradiation distance is 25~30cm) Sterilize for 30min, turn over and continue irradiation for 3...

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Abstract

The invention discloses a method for improving dried orange peel flavonoid content by using aspergillus flavus. According to the method, dried orange peel is processed by using the aspergillus flavus. By using the aspergillus flavus to process the dried orange peel, total flavonoid content and content of four kinds of flavonoid of naringin, aurantiamarin, nobiletin and tangeretin in the dried orange peel can be improved in a short time, the increase of antioxidant activity of the dried orange peel can be promoted, and a good application prospect is realized.

Description

technical field [0001] The invention relates to a method for increasing the flavonoid content of tangerine peel by using Aspergillus flavus. Background technique [0002] Tangerine peel is the dry and mature peel of Citrus reticulata Blanco and its cultivars of the Rutaceae plant. It was first recorded in "Shen Nong's Materia Medica" and listed as top grade. It is commonly used for both medicine and food, with a wide range of uses and a large dosage. Tangerine peel is fragrant and slightly bitter. It is mainly used to treat symptoms such as distension and fullness in the abdomen, lack of appetite, vomiting and diarrhea, and cough with excessive phlegm. Pharmacological studies have shown that tangerine peel has good medicinal value in cardiovascular system, immunity, anti-oxidation and anti-tumor. [0003] The chemical composition of tangerine peel is mainly based on flavonoids, and also contains substances such as volatile oil, limonoids, and alkaloids. Flavonoids have th...

Claims

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Application Information

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IPC IPC(8): A61K36/752A61P39/06
CPCA61K36/752A61K2236/19
Inventor 刘友平陈鸿平王福张鑫陈林
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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