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A high-efficiency lignocellulose-degrading bacteria k20 and its application

A lignocellulosic and high-efficiency degrading bacteria technology, which is applied in the direction of bacteria, microorganisms, and methods based on microorganisms, can solve the problems of low enzyme activity and achieve stable enzyme activity, rapid value-added, and stable enzyme production.

Active Publication Date: 2018-11-23
北京中农国泰科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the use of fungi to degrade lignocellulose generally has the problem of low enzyme activity

Method used

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  • A high-efficiency lignocellulose-degrading bacteria k20 and its application
  • A high-efficiency lignocellulose-degrading bacteria k20 and its application
  • A high-efficiency lignocellulose-degrading bacteria k20 and its application

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specific Embodiment approach 1

[0024] Specific embodiment one: a lignocellulosic material efficient degrading bacterium K20 of this embodiment, it is enterobacter hormaechei (Enterobacter hormaechei) K20, is preserved in the common microorganism center of China Committee for Microorganism Culture Collection, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city, with a preservation date of December 12, 2014, and a preservation number of CGMCC No.10163.

[0025] Enterobacter hormaechei (Enterobacter hormaechei) K20 of the present embodiment is a Gram-negative bacterium, and the bacterial cell shape of this strain is rod-shaped, and the bacterial cell size is 0.51~0.57×1.2~1.5 μm, does not form spores, and has no flagella and pods Membrane; forms round, opaque, milky-white colonies with smooth protrusions and neat edges on LB medium (such as figure 1 shown).

[0026] The results of the physiological and biochemical reactions of the strain included 18 i...

specific Embodiment approach 2

[0087] Embodiment 2: The application of a lignocellulosic substance-degrading bacteria in this embodiment, which is used to degrade lignocellulosic substances.

[0088] Bacterial strain of the present invention is carried out following functional detection:

[0089] Enterobacter hormaechei (Enterobacter hormaechei) K20 of the specific embodiment was tested for weight loss rate and lignin, cellulose and hemicellulose degradation of bagged fungus waste and corn stalks to verify its unique functions. details as follows:

[0090] 1. Determination of weight loss rate of bagged fungus waste and corn stalks

[0091] 1.1 Corn stalk powder

[0092] Corn stalks were taken from the experimental field of the laboratory in Hulan Campus, Heilongjiang University, Harbin City, Heilongjiang Province in October 2012, dried, crushed through a 40-mesh sieve, and used for later use.

[0093] 1.2 bags of fungus waste

[0094] Bags of fungus waste were provided by Mudanjiang Branch of Heilongjia...

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Abstract

The invention relates to a lignocellulos substance high-efficiency degrading bacterium K20 and application thereof. The lignocellulos substance high-efficiency degrading bacterium K20 is Enterobacter hormaechei K20 and was preserved with preservation number CGMCC No.10163 on December 12, 2014 by China General Microbiological Culture Collection Center. The bacterium is used for degrading lignocellulos substances. The strain provided by the invention has the advantages of quick proliferation, strong applicability and wide application; a weight-loss ratio of bagged agaric wastes after being fermented by Enterobacter hormaechei K20 is above 23 percent and a weight-loss ratio of corn stalks after being fermented by Enterobacter hormaechei K20 is above 27 percent.

Description

technical field [0001] The invention relates to a high-efficiency degradation bacterium K20 of lignocellulosic substances and its application. Background technique [0002] my country is a large agricultural country, and the amount of agricultural waste generated is extremely huge. Due to the limitations of economic benefits and technology popularization, most of the agricultural waste is extensively and inefficiently used and the state of idleness is serious, resulting in waste of resources and environmental pollution. Waste has become China's largest source of pollution. However, there is a large amount of lignocellulose in agricultural waste, which needs to be developed and utilized urgently. [0003] Lignocellulose is the second most abundant organic renewable resource after cellulose in nature, and it is also one of the most difficult components for microorganisms to degrade. In recent years, some lignin-degrading fungi have begun to be applied in practice, but further...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 丁仕波王鲲鹏杨峰山孙永帅吴奇杨微刘春光王庆华魏才强曲金玲张慧于文全
Owner 北京中农国泰科技有限公司
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