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Tissue Culture Rapid Propagation Method of Macadamia Nut

A technology of tissue culture and nuts, which is applied in the field of tissue culture and rapid propagation of macadamia nuts, which can solve problems such as difficulty in rooting explants, achieve the effects of shortening the time of tissue culture, improving the ability of proliferation, and improving the quality of varieties

Active Publication Date: 2017-08-25
广西合山市润鸿农业技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Still another object of the present invention is to provide a liquid medium, which is atomized by the cultivation bed of the present invention, absorbs moisture and nutrients in the liquid medium through the 2 layers of flax fiber cloth at the bottom of the solid medium, thereby inducing rooting, And increase the rooting rate, thereby solving the problem of difficult rooting of macadamia nut explants

Method used

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  • Tissue Culture Rapid Propagation Method of Macadamia Nut

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Embodiment 1

[0034] The present invention provides a tissue culture rapid propagation method of macadamia nuts, comprising:

[0035] Step A, intercepting 4 cm long stem segments containing buds from adult macadamia trees as explants, each stem segment contains an axillary bud, and the explants are sterilized;

[0036] Step B, insert the explants obtained in step A into the induction medium and carry out induction culture for 20 days, the culture temperature is 23°C, the light intensity is 2000Lux, and the light is 13h every day. The formula of the induction medium is: 1 / 2MS, 6-benzyl adenine 0.2mg / L, gibberellin 0.2mg / L, vitamin B 1 0.45mg / L, agar 7g / L and sucrose 25g / L;

[0037] Step C, cutting the new long axillary bud seedlings cultivated in step B into stem segments with only one axillary bud under aseptic conditions, inserting into the rooting medium and cultivating them at a culture temperature of 25°C, including three stages in turn : In the first stage, the light intensity is 2...

Embodiment 2

[0050] A tissue culture rapid propagation method of macadamia nuts, comprising:

[0051] Step A, intercepting 4 cm long stem segments containing buds from adult macadamia trees as explants, each stem segment contains an axillary bud, and the explants are sterilized;

[0052] Step B, insert the explants obtained in step A into the induction medium for induction culture for 20-24 days, the culture temperature is 24°C, the light intensity is 2100Lux, and the light is 14h every day. The formula of the induction medium is: 1 / 2MS, 6-benzyl adenine 0.3mg / L, gibberellin 0.25mg / L, vitamin B 1 0.50mg / L, agar 7g / L and sucrose 25g / L;

[0053] Step C, cutting the new long axillary bud seedlings cultivated in step B into stem segments with only one axillary bud under aseptic conditions, inserting into the rooting medium and cultivating them at a culture temperature of 26°C, including three stages in turn : In the first stage, the light intensity is 2300Lux, and the light is 15h per day f...

Embodiment 3

[0065] A tissue culture rapid propagation method of macadamia nuts, comprising:

[0066] Step A, intercepting 4 cm long stem segments containing buds from adult macadamia trees as explants, each stem segment contains an axillary bud, and the explants are sterilized;

[0067] Step B, insert the explants obtained in step A into the induction medium for induction culture for 24 days, the culture temperature is 25°C, the light intensity is 2200Lux, and the light is 15h every day. The formula of the induction medium is: 1 / 2MS, 6-benzyl adenine 0.4mg / L, gibberellin 0.3mg / L, vitamin B 1 0.55mg / L, agar 7g / L and sucrose 25g / L;

[0068] Step C, cutting the new long axillary bud seedlings cultivated in step B into stem segments with only one axillary bud under aseptic conditions, inserting into the rooting medium and cultivating them at a culture temperature of 27°C, including three stages in turn : In the first stage, the light intensity is 2400Lux, and the light is 16h per day for ...

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Abstract

The invention discloses a method for tissue culture rapid propagation of macadamia nuts, comprising: cutting a 4cm-long stem section containing buds from an adult tree of macadamia nuts as an explant, and disinfecting the explant; Carry out induction culture for 20-24 days, the temperature is 23-25°C, the light intensity is 2000-2200Lux, and the light is 13-15h per day; the new long axillary buds cultivated are cut into stems with only one axillary bud under aseptic conditions , inserted into the rooting medium and cultivated at a temperature of 25-27°C, including three stages in turn: the first stage, placed in 2200-2400Lux, 14-16h per day, and cultivated for 12-14 days; the second stage, cultivated in the dark 8‑10 days; in the third stage, culture at 2800‑3000 Lux for 18 hours per day until rooting. The invention solves the problems of difficult rooting and low rooting rate of macadamia nut tissue culture.

Description

technical field [0001] The invention relates to the field of tissue culture of macadamia nuts. More specifically, the present invention relates to a tissue culture rapid propagation method of macadamia nuts. Background technique [0002] Macadamia nuts, also known as Queensland chestnuts and macadamia nuts. Its kernels are rich in unsaturated fatty acids, proteins, vitamins, etc., with high nutritional value and unique flavor, known as the "king of dried fruits". The market prospect of macadamia nuts is promising, with good ecological and economic benefits. It is an edible nut with high oil content, rich nutrition and unique flavor. It has been in short supply in the international market. [0003] Macadamia nut seedling raising methods include seed seedling raising, cutting seedling raising, grafting seedling raising and plant tissue culture seedling raising method. Due to the poor genetic stability of macadamia seed breeding, seedlings are rarely propagated by single see...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 凌华彪
Owner 广西合山市润鸿农业技术有限公司
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