A device and method for in-situ automatic enrichment of microorganisms applied to the whole sea depth

Abstract

The invention discloses an in-situ automatic gathering and fixing device applied to microorganisms at all sea depths. The device comprises a cabin body, an electronic reversing valve, a power pump, a control circuit, a filter membrane cabin and a reagent bag, wherein the reagent bag is filled with microorganism fixing liquid; a filter membrane is mounted in the filter membrane cabin; a power supply serial port is formed in one end of the cabin body; the other end of the cabin body is fixedly connected with the filter membrane cabin; a bracket is arranged in the cabin body; the electronic reversing valve and the power pump are connected onto the bracket; the inlet of the electronic reversing valve is connected with the reagent bag and the outside of the cabin body in parallel through pipelines; the outlet of the electronic reversing valve is communicated with the inlet of the power pump through a pipeline; the outlet of the power pump is communicated with the filter membrane cabin through a pipeline; a water discharging non-return valve is arranged on the filter membrane cabin. After the gathering is finished, the inlet of the electronic reversing valve and a microorganism fixing liquid passage of the reagent bag are opened, and the microorganism fixing liquid enters the filter membrane cabin along the pipeline to displace sea water in the filter membrane cabin and fix microorganisms on the filter membrane.

Description

technical field [0001] The invention relates to the technical field of deep-sea automatic mechanical devices, in particular to an in-situ automatic enrichment and fixing device and method for microorganisms applied to the whole sea depth. Background technique [0002] At present, in the field of deep-sea scientific microbial research, how to obtain in-situ samples is an indispensable part of the deep-sea research process. In recent years, considerable research has been carried out on the community composition and functional characteristics of deep-sea microorganisms, but there are still many deficiencies. Which effective sampling method to use to truly reflect the activity state and gene expression status of deep-sea microorganisms is one of the main issues. [0003] Compared with DNA molecules, RNA is very easy to degrade, and its half-life is generally only a few minutes, which poses a great challenge to how to obtain transcriptome data that truly reflect the in situ expre...

AI Technical Summary

Problems solved by technology

For deep-sea microbial sampling, the conventional method is to collect seawater samples through Niskin sampling bottles, and return the samples to the ship for filtration, collection and fixed treatment. This sampling method has many shortcomings: On the one hand, the time from sampling to recovery to the ship The interval is generally more than tens of minutes, and this time period will affect the transcriptome of microorganisms. On the other hand, the sample recovery process involves great changes in pressure, temperature and dissolved oxygen, which become important factors affecting the expression of microbial transcriptomes.

These factors will cause the collected deep-sea microbial samples to fail to truly reflect their in situ expression status

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