Method for removing ammonia nitrogen and total nitrogen in tail water with bacterial agent strengthened subsurface flow wetland
A technology for subsurface wetlands and tail water, applied in chemical instruments and methods, water pollutants, biological water/sewage treatment, etc., can solve the problem of low denitrification effect, improve the removal effect, save investment, and slow down the replacement of fillers The effect of frequency
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[0018] 1. Preparation of B8 bacterial agent
[0019] Use a sterilized inoculating pen to pick 1 environmentally friendly Pseudomonas putida B8 hidden in the slant medium, and inoculate it in a 100 mL conical flask containing 50 mL DNJPU medium pH 6.5 after high temperature steam sterilization, and place it on a shaker. (Set culture temperature 30℃, shaker speed 120r / min) Shake culture for 20h to obtain B8 preparation (the number of viable bacteria is 6.25×10 7 ~8.55×10 7 cfu / mL).
[0020] 2. Bacteria inoculation in constructed wetlands
[0021] The tail water of the urban sewage treatment plant that meets the "Urban Sewage Treatment Plant Pollutant Discharge Standard" (GB18918-2002) level B water quality standard is treated as wastewater to be treated in the subsurface wetland system for treatment. The prepared B8 formulation was added to the horizontal subsurface flow constructed wetland system, and the specific operation was as follows: the B8 formulation was added to 0.2% of the ...
Example Embodiment
[0025] Example 1
[0026] This embodiment treats 12m of tail water from a sewage treatment plant 3 / d. After the inoculation operation of the constructed wetland (the porosity of the wetland filler is 30.75%) is completed, the B8 formulation enhanced subsurface flow wetland system is operated for 6 days, and its denitrification effect is monitored. The specific operation is: adopt the operation mode for water intake-wetland deep purification The 24h-empty constructed wetland system deeply purifies the tail water of the sewage treatment plant.
[0027] Table 1 Construction, operation and treatment water quality of the undercurrent wetland system
[0028]
Example Embodiment
[0029] Example 2
[0030] This embodiment treats 21m of tail water from a sewage treatment plant 3 / d. After the inoculation operation of the constructed wetland (the porosity of the wetland filler is 34.34%) is completed, the denitrification effect of the B8 formulation enhanced subsurface flow wetland system is monitored for 19 days, and the specific operation is: the operation mode is the water intake-wetland deep purification The 24h-empty constructed wetland system deeply purifies the tail water of the sewage treatment plant.
[0031] Table 2 Construction, operation and treatment water quality of the undercurrent wetland system
[0032]
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