Living plant ascorbic acid detection method based on microelectrode biosensor

A biosensor, ascorbic acid technology, applied in the field of detection, can solve problems such as static detection only, irreversible damage to samples, complex sample processing, etc., and achieve the effect of simple detection conditions and portable detection equipment

Active Publication Date: 2016-04-06
BEIJING RES CENT FOR INFORMATION TECH & AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a rapid detection method of ascorbic acid in living plants ba

Method used

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  • Living plant ascorbic acid detection method based on microelectrode biosensor
  • Living plant ascorbic acid detection method based on microelectrode biosensor
  • Living plant ascorbic acid detection method based on microelectrode biosensor

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Experimental program
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Effect test

Embodiment 1

[0035] Such as figure 1 , the microelectrode array is prepared on the silicon wafer substrate 1 by etching technology and electrodeposition technology, with a length of 5 cm and a thickness of 1.5 mm. The electrode 4 has a sharp point facing outward from the silicon chip substrate 1, and the other end is wider, on which a connecting element 5 is arranged (one electrode is connected to a corresponding connecting element). Among them, Ag / AgCl is the reference electrode, platinum is the counter electrode, and the modified gold electrode is the working electrode. During detection, an electrochemical workstation is used to connect the connection element 5 in the microelectrode array, and the current is detected by cyclic voltammetry on the electrochemical workstation, and then the current data is converted into concentration data, and the data is collected by the signal acquisition module.

[0036] see figure 2 , the modification steps of the gold electrode are as follows:

[0...

Embodiment 2

[0042] Glucose-phosphate buffer (pH=7.4) solutions with concentrations of 0, 1, 2, 4, 6, 8, and 10 mmol / L were prepared respectively, and the prepared microelectrodes were used for cyclic voltammetry detection (voltage -0.2 to 1.0V, scanning Speed ​​100mV / s), obtain the curve ( image 3 ), make the microelectrode working curve, the linear equation is i=1.308+0.0515c (the unit of current i is μA, the unit of concentration c is mmol / L), and the linear range can reach 1-10mmol / L.

[0043] After cleaning the microelectrode prepared in Example 1, first detect three parts of standard concentration (2, 5, 8mmol / L) ascorbic acid solution for electrochemical calibration, and the biosensor system will automatically adjust the deviation to meet the working curve and slope deviation through calculation. Within 15%, the microelectrodes were determined to be functioning properly.

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Abstract

The invention brings forward a living plant ascorbic acid detection method based on a microelectrode biosensor. The method comprises the following steps: S1, forming the microelectrode biosensor by using a microelectrode array which is connected to a signal acquisition module and an electrochemical workstation; S2, testing an ascorbic acid standard solution by using the microelectrode biosensor to obtain a mathematical model on the relation between ascorbic acid concentration and current signals; and S3, inserting the microelectrode biosensor into a living plant to be tested to obtain response of the living plant to an input electric signal, and calculating concentration of ascorbic acid in the living plant sample to be tested according to the mathematical model in the step S2. According to the invention, detection equipment is portable, detection condition is simple, detection can be operated in the field, and continuous detection can be realized.

Description

technical field [0001] The invention belongs to the detection field, and in particular relates to a method for detecting ascorbic acid by using a biosensor. Background technique [0002] Ascorbic acid is a high-abundance small-molecule antioxidant substance commonly found in plant tissues. It can directly react with active oxygen such as singlet oxygen, superoxide radical, hydrogen peroxide and hydroxyl radical, so it plays an important role in plant resistance to oxidative stress. At the same time, ascorbic acid is also the reaction substrate of some key enzymes, so ascorbic acid plays an important role in the process of plant growth and development and plant response to environmental stress, it is necessary to detect and monitor the content of ascorbic acid in plant tissues. [0003] At present, the classic iodometric method, liquid chromatography-mass spectrometry, capillary electrophoresis, flame atomic absorption spectrometry, and spectrophotometry are commonly used in...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 赵春江胡叶何璐璐王晓冬任鹏周航侯佩臣宋鹏高权陈泉
Owner BEIJING RES CENT FOR INFORMATION TECH & AGRI
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