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Test strip for quantitatively detecting prostate-specific antigen as well as preparation method and test paper card

A prostate-specific and quantitative detection technology, applied in the field of in vitro test strip detection, can solve problems such as easy errors, achieve high accuracy, improve fluorescence intensity, and reduce quenching effects

Inactive Publication Date: 2016-04-13
JIANGSU INST OF NUCLEAR MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the detection test kits used in the prior art for detecting prostate specific antigen can only detect total or free prostate specific antigen, but the free and total prostate specific antigen in the calculation sample liquid The technical defect that errors are prone to occur when the ratio of sex antigens; and then provide a test strip for simultaneous detection of free and total prostate specific antigen and a test strip card using it

Method used

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  • Test strip for quantitatively detecting prostate-specific antigen as well as preparation method and test paper card
  • Test strip for quantitatively detecting prostate-specific antigen as well as preparation method and test paper card
  • Test strip for quantitatively detecting prostate-specific antigen as well as preparation method and test paper card

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Such as figure 1 As shown, a test strip for simultaneous detection of free and total prostate-specific antigen includes a base plate 1 and absorbent paper 3, nitrocellulose membrane 2, and binding pad 5 sequentially adhered to the base plate 1 along the length direction of the base plate 1 and sample pad 4;

[0044] The nitrocellulose membrane 2 is adhered to the middle part of the bottom plate 1, and the two ends overlap with the absorbent paper 3 and the binding pad 5 respectively; the other end of the binding pad 5 partially overlaps with the sample pad 4;

[0045] A first detection zone 7, a second detection zone 8 and a quality control zone 6 are sequentially arranged on the nitrocellulose membrane 2 at intervals;

[0046] The surface of the binding pad 5 is sprayed with anti-compound prostate-specific antigen antibody and anti-free prostate-specific antigen antibody, and both the anti-compound prostate-specific antigen antibody and the anti-free prostate-specific...

Embodiment 2

[0051] A method for preparing a test strip for simultaneously detecting free and total prostate-specific antigens, comprising the following steps:

[0052] (1) Antibody pretreatment:

[0053] Dialyze the commercial composite PSA scratching antibody and the free PSA scratching antibody overnight at 4°C with 0.05mol / L phosphate buffer solution with a pH of 7.2-7.6;

[0054] (2), preparation of bonding pad 5:

[0055] Add carbodiimide (EDC) (the final concentration of EDC is 20mmol / L) and step (1) pretreated composite prostate-specific antigen delimiting antibody in the nanometer microsphere solution of lanthanide rare earth ion, nanometer microsphere The mass ratio to the pretreated antibody is 50:1, react at room temperature for 2 hours, centrifuge, remove the supernatant, add sample diluent (0.05 mol / L of BSA containing 1% mass fraction, phosphoric acid at pH 7.2 Salt buffer solution) until the microsphere concentration is 1.0ug / L, stand-by;

[0056] Add carbodiimide (EDC) ...

Embodiment 3

[0062] Such as figure 2 As shown, a test paper card for quantitative detection of prostate specific antigen comprises a test paper strip for quantitative detection of prostate specific antigen and a casing covering the test strip; the casing includes a base and a card cover 9, and the card cover 9 is provided with a Expose the local area observation port 11 and sample addition port 10 of the test strip; wherein, the sample addition port 10 is located above the sample pad 4 to expose part or all of the sample pad 4 area, and the observation port 11 is located on the nitrocellulose The upper part of the prime film 2 to expose the first detection zone 7 , the second detection zone 8 and the quality control zone 6 . Both the base and the card cover 9 are made of plastic.

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Abstract

The invention discloses a test strip for quantitatively detecting contents of a total prostate specific antigen and a free prostate specific antigen simultaneously through adopting a time-resolved fluorescent immunochromatographic method as well as a preparation method and a test paper card. The test strip comprises a bottom board, absorbent paper, a nitrocellulose membrane, a combining pad and a sample pad, wherein the absorbent paper, the nitrocellulose membrane, the combining pad and the sample pad are sequentially adhered to the bottom board; a first inspection band of an fPSA membrane scratching antibody, a second inspection band of a complex PSA membrane scratching antibody and a quality control band of a rabbit antimouse polyclonal antibody are sequentially arranged on the nitrocellulose membrane at intervals in parallel, the surface of the combining pad is coated with a composite prostate-specific antigen antibody and a free prostate specific antigen antibody, and both the composite prostate-specific antigen antibody and the free prostate specific antigen antibody are coupled with nano microspheres with lanthanum rare earth ions, so that the high detection result accuracy and small detection result errors of the tPSA and the fPSA in same sample liquid are guaranteed, and great convenience is provided for clinical application.

Description

technical field [0001] The invention relates to the technical field of in vitro test paper detection, in particular to a test strip for simultaneously quantitatively detecting the contents of total prostate specific antigen and free prostate specific antigen by using a time-resolved fluorescence immunochromatography method, a preparation method and a test paper card. Background technique [0002] Prostate Specific Antigen (PSA) is a proteolytic enzyme produced by prostate epithelial cells. Under normal physiological conditions, PSA mainly exists in semen, and its concentration in semen is 1 million times higher than that in serum. There are two forms of PSA in the blood circulation. The composite PSA combined with proteolytic enzyme inhibitors accounts for more than 85%, the free PSA (fPSA) accounts for about 15%, and the total prostate-specific antigen (tPSA) is composite PSA and free PSA. Sum. PSA can be elevated in prostatitis, benign prostatic hyperplasia, prostatic isc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N33/574G01N33/558G01N33/531G01N33/58
CPCG01N33/573G01N33/531G01N33/558G01N33/57434G01N33/57488G01N33/582G01N2333/914
Inventor 黄飚张艺张珏周彬郭明明范俊邓黎莉朱岚
Owner JIANGSU INST OF NUCLEAR MEDICINE
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