In-vitro rapid propagation technology of viburnum sargentii koehne

Abstract

The invention belongs to the field of plant tissue culture, and provides an in-vitro rapid propagation technology of viburnum sargentii koehne. According to the technology, tender stem segments of the viburnum sargentii koehne serve as explants, MS serves as a basic culture medium, plant growth regulators 6-BA and IBA are added to induce axillary buds to germinate and grow, multiplication of subculture, rooting induction and acclimatization and transplanting are conducted, and a large number of regeneration plants with consistent heredity can be obtained within a short time. The in-vitro rapid propagation technology provides a technological support for rapid propagation of an improved variety of the viburnum sargentii koehne, the demand of China for the viburnum sargentii koehne is also met, and important practical significance is achieved for accelerating comprehensive development and utilization of the viburnum sargentii koehne.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a technique for in vitro rapid propagation of Viburnum viburnum. Background technique [0002] Viburnum sargentii Koehne (Viburnum sargentii Koehne), also known as Viburnum sargentii Koehne, is a deciduous shrub of the genus Viburnum in the family Caprifoliaceae. It has strong adaptability to the environment, is resistant to cold and drought, and has no strict requirements on the soil. Strong germination, resistant to pruning, well-developed root system, easy to transplant and survive. Viburnum viburnum is a beautiful tree, with green leaves in spring, blooming flowers in summer, red fruits in autumn, crystal clear, and red fruits in winter. It is an excellent greening tree species that can be viewed in flowers, fruits and leaves. [0003] The fruit of Viburnum japonicus is rich in nutrition, containing high amino acids, vitamins and trace elements. The leaves a...