Check patentability & draft patents in minutes with Patsnap Eureka AI!

Extraction method of camellia seed flavone

An extraction method and technology of seed flavonoids, which are applied in the direction of pharmaceutical formulas, skin care preparations, medical preparations containing active ingredients, etc., can solve the problems of difficult denaturation of active ingredients, long time-consuming, and less extraction of natural plant active ingredients, etc., to achieve The effect of efficient extraction methods

Inactive Publication Date: 2016-04-20
SHANGHAI FOREST CABIN BIOLOGICAL TECH
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process takes a long time and is relatively expensive
[0004] The cloud point extraction method has simple operation steps, wide application range, high extraction yield, no use of volatile organic solvents, recyclable surfactant, low extraction temperature, low denaturation of active ingredients during the extraction process, economical, safe, and It is widely used in the field of analysis due to the characteristics of easy amplification and environmental friendliness, but it is relatively rare in the extraction of natural plant active ingredients.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extraction method of camellia seed flavone
  • Extraction method of camellia seed flavone
  • Extraction method of camellia seed flavone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This embodiment relates to a method for extracting flavonoids in the seeds of Camellia genus based on the cloud point extraction method, comprising the following steps:

[0034] Step 1: Extraction step

[0035] According to the solid-to-liquid ratio g / mL of 1:30, weigh the defatted camellia plant seed pulverization, add a nonionic surfactant polyethylene glycol-8 laurate solution with a concentration of 30 g / L, and extract with the aid of ultrasonic waves for 30 minutes , and filtered to obtain a solid filter residue and an extract containing camellia seed flavonoids.

[0036] Step 2: Cloud point phase separation extraction step

[0037] Add sodium chloride to the camellia seed flavonoid extract obtained in the extraction step to adjust the ionic strength to 1.0 mol / L, then place the extract in a constant temperature water bath at a temperature of 50° C., and keep it in a constant temperature balance for 40 minutes, then let stand or centrifuge until The phase separat...

Embodiment 2

[0043] This embodiment relates to a method for extracting flavonoids in the seeds of Camellia genus based on the cloud point extraction method, comprising the following steps:

[0044] Step 1: Extraction step

[0045] Take by weighing 100g of the Camellia seeds harvested in the same year, pulverize with a universal pulverizer, pass through a standard screen with a 0.5mm aperture, degrease twice with petroleum ether, evaporate the residue to dry the solvent, and set aside;

[0046]According to the solid-to-liquid ratio g / mL of 1:30, weigh the defatted camellia plant seed pulverization, add a nonionic surfactant polyethylene glycol-8 laurate solution with a concentration of 40 g / L, and ultrasonically assist extraction for 35 minutes , and filtered to obtain a solid filter residue and an extract containing camellia seed flavonoids.

[0047] Step 2: Cloud point phase separation extraction step

[0048] Add sodium chloride to the camellia seed flavonoid extract obtained in the ex...

Embodiment 3

[0052] This embodiment relates to a method for extracting flavonoids in the seeds of Camellia genus based on the cloud point extraction method, comprising the following steps:

[0053] Step 1: Extraction step

[0054] Take by weighing 100g of the Camellia seeds harvested in the same year, pulverize with a universal pulverizer, pass through a standard screen with a 0.5mm aperture, degrease twice with petroleum ether, evaporate the residue to dry the solvent, and set aside;

[0055] According to the solid-to-liquid ratio g / mL of 1:30, weigh the defatted camellia plant seed pulverization, add a non-ionic surfactant polypropylene glycol-3 laurate solution with a concentration of 60 g / L, ultrasonically assisted extraction for 40 minutes, and filter , to obtain the solid filter residue and the extract containing camellia seed flavonoids.

[0056] Step 2: Cloud point phase separation extraction step

[0057] Add sodium chloride to the camellia seed flavonoid extract obtained in the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Cloud pointaaaaaaaaaa
Ionic strengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses an extraction method of camellia seed flavone. The extraction method comprises steps as follows: smashed defatted camellia plant seeds are taken, 20-60 g / L of a surfactant solution is added, a mixture is subjected to ultrasonic-assisted extraction for 30-40 min and is filtered, and solid filter residues and a camellia seed flavone contained extraction solution are obtained; an electrolyte is added to the extraction solution, ion strength is regulated to be 0.5-1.5 mol / L, the extraction solution is subjected to constant-temperature balancing for 20-60 min at the balancing temperature of 40-80 DEG C and then is left to stand or centrifuged, an aqueous phase and a surfactant enriched phase are separated; the surfactant enriched phase is subjected to vacuum drying and water removing under the condition of the temperature lower than or equal to 50 DEG C, and camellia seed flavone is obtained. Compared with a traditional solvent extraction method, the extraction method has the advantage that the extraction efficiency is improved by about 1.5 times; the extraction technology does not utilize a volatile organic solvent, does not influence the environment and is environment-friendly, the extraction method is fast, simple and effective, and the acquired extract can be taken as an efficacy type surfactant and is directly applied to cosmetic formulation.

Description

technical field [0001] The invention relates to plant extraction, in particular to a method for extracting camellia seed flavonoids. Background technique [0002] At present, in the research on Camellia family plants at home and abroad, the research reports on camellia oil and its tea seed cake are the most, and there are many researches and applications on camellia oil, focusing on the research and utilization of camellia plant camellia seed oil. The development of tea saponin in its tea seed cake, but the research and utilization of camellia seed cake is very little. Studies have found that the residue of extracted camellia oil contains strong antioxidant active ingredients, which are reported to be flavonoids. [0003] In the development and research of tea seed cake, there are some researches on the production process of its flavonoid components. For example: CN101899070A discloses "a preparation method for rapid separation of flavonoid glycosides in camellia oleifera ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K8/49A61Q19/00A61K8/92A61Q5/02
CPCA61K8/498A61K8/922A61K2800/10A61K2800/596A61K2800/82A61Q5/006A61Q5/02A61Q19/00
Inventor 刘学高宏旗陈贤
Owner SHANGHAI FOREST CABIN BIOLOGICAL TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com