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Lactobacillus mucosae with anti-aging function

A technology of Lactobacillus mucosae and strains, applied in the field of microorganisms, can solve problems such as no reported free radical scavenging effect, and achieve the effects of prolonging the lifespan of mice, alleviating adverse symptoms, and good acid resistance and bile salt resistance.

Active Publication Date: 2016-04-20
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, no strains of mucosal Lactobacillus species have been reported to scavenge free radicals and delay aging

Method used

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  • Lactobacillus mucosae with anti-aging function
  • Lactobacillus mucosae with anti-aging function
  • Lactobacillus mucosae with anti-aging function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation and Identification of Lactobacillus Lactobacillus ZDY308

[0031] Lactobacillus Lactobacillus ZDY308 was isolated from feces of healthy and long-lived elderly. Dissolve 1 g of feces in 10 mL of 10% peptone solution, fully dissolve, filter the residue with filter paper, centrifuge the filtrate at 5000 rpm for 10 min, discard the supernatant, collect the precipitate, and resuspend in 1 mL of 10% peptone water. Inoculate into MRS liquid medium at 1% and culture overnight. The culture solution was serially diluted 10 times, and the serial dilution was 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 and 10 -9 , 1 mL of each gradient was evenly spread on the agar plate of MRS broth, and cultured anaerobically at 37°C for 48h. Pick a single colony, separate it by streaking on a plate, carry out repeated purification and culture, and observe the colony morphology with Gram staining method to select Gram-positive bacteria. After the isolate...

Embodiment 2

[0036] Example 2: Tolerance of bacterial strains to oxygen stress

[0037] Inoculate the freshly cultivated bacterial strain to a concentration of 0.4mmol / LH at a ratio of 1%. 2 o 2 In the MRS liquid medium, cultured anaerobically at 37°C for 8h, and measured the OD at 0h and 8h respectively 630 , to detect strains against H 2 o 2 tolerance.

[0038] The results showed that: ΔOD>0.9, with strong tolerance.

Embodiment 3

[0039] Embodiment 3: strain and fermentation supernatant scavenging DPPH free radical ability

[0040] According to the inoculum size of 1%, it was inserted into the MRS medium, cultured anaerobically at 37°C for 18h, centrifuged at 6000r / min for 10min, and the fermentation supernatant and cells were collected respectively. The cells were washed twice with PBS (pH7.2) solution, and then resuspended in PBS solution until the number of lactic acid bacteria reached 10. 9 cfu / mL. Fermentation supernatant and resuspended bacteria were used as samples for free radical scavenging test.

[0041] Add 2mL DPPH absolute ethanol solution (0.2mmol / L) to 2mL sample and mix well, react in the dark at room temperature for 30min, centrifuge at 6000r / min for 10min, take the supernatant and measure the absorbance value A at 517nm i ; In the blank group, an equal volume of absolute ethanol was used instead of DPPH absolute ethanol solution A o , the control group replaced the sample solution A...

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Abstract

The invention discloses novel lactobacillus mucosae CCTCC NO:M 2015581 with high anti-oxidation activity. The lactobacillus mucosae is separated from faeces of healthy and longevous old people, is gram-positive bacteria, has very high free radical scavenging capacity, can improve the activity of antioxidant enzymes in a living body, can delay aging, has excellent bile salt resistance and can be developed into a micro-ecological microbial agent and used as a food additive to improve the gastrointestinal tract of a host.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a new strain of Lactobacillus mucosae and its beneficial function, especially the function of removing free radicals and delaying aging. Background technique [0002] As a virtual "organ", the intestinal flora affects the development, growth, nutrition and health of the human body, and is even closely related to metabolic diseases (irritable bowel syndrome, inflammatory bowel disease, cardiovascular disease, diabetes, obesity, etc.) , the correlation between intestinal flora and human health and even longevity. [0003] Gaotian Village, Liuyang City, Hunan Province has a relatively closed geographical location. The villagers not only live a long life, but also generally have no chronic diseases. In stark contrast, the surrounding area has no longevity phenomenon, and diseases such as cardiovascular disease, chronic gastroenteritis and cancer occur from time to time. [0004] Lactic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23L19/20A23L2/38A23L19/00A23L33/135A61K35/747A61K8/99A61P1/00A61P39/06A61Q19/08C12R1/225
Inventor 徐锋魏华蔚晓敏杨栋吴姚平裘梁王登远
Owner NANCHANG UNIV
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