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A kind of immobilization method of anaerobic bacteria

A technology of anaerobic bacteria and calcium chloride, applied in chemical instruments and methods, fixed on/in organic carriers, water/sludge/sewage treatment, etc., can solve problems such as easy adhesion, and achieve a solution to adhesion , Realize the effect of large-scale equipment production

Active Publication Date: 2018-12-21
POTEN ENVIRONMENT GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The embodiment of the invention discloses a method for immobilizing anaerobic bacteria, which solves the problem that the usual embedding method is prone to adhesion during the immobilization of anaerobic bacteria, and can realize large-scale equipment production

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  • A kind of immobilization method of anaerobic bacteria

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Embodiment 1

[0048] (1) Prepare curing agent: add boric acid to 500mL distilled water and dissolve to obtain saturated boric acid solution, weigh 6gCaCl 2 , 0.6g talcum powder, join in the saturated boric acid solution, make curing agent.

[0049] (2) Preparation of growth factor solution: 10.0 mg each of vitamin B1, vitamin B6, and nicotinic acid, 3 g of glucose, and 0.15 g of ammonium tartrate were added to 500 mL of water to prepare a growth factor solution.

[0050] (3) Preparation of immobilized anaerobic bacteria particles: Add 6 g of polyvinyl alcohol, 2 g of sodium alginate and 10 g of zeolite powder with a particle size of 200 mesh to 200 mL of growth factor solution, heat to 50 ° C, and continuously stir at a speed of 70 rpm for 1 hour. A mixed slurry is prepared. After the temperature of the mixed slurry was lowered to 30° C., 10 mL of anaerobic bacteria was added to the mixed slurry, and the stirring was continued to prepare a mixed slurry containing anaerobic bacteria. Use a...

Embodiment 2

[0052] (1) Prepare curing agent: add boric acid to 500mL distilled water and dissolve to obtain saturated boric acid solution, weigh 55gCaCl 2 , 5g talcum powder, join in the saturated boric acid solution, make curing agent.

[0053] (2) Preparation of growth factor solution: 15.0 mg each of vitamin B1, vitamin B6, and nicotinic acid, 4 g of glucose, 0.15 g of ammonium tartrate, and 5 mL of 25% ethanol solution were added to 500 mL of distilled water to prepare a growth factor solution.

[0054] (3) Preparation of immobilized anaerobic bacteria granules: Add 20 g of polyvinyl alcohol, 0.2 g of sodium alginate and 3.3 g of activated carbon powder with a particle size of 325 mesh to 200 mL of growth factor solution, heat to 120 ° C, and continuously stir at 80 rpm for 50 min , to prepare a mixed slurry. After the temperature of the mixed slurry was lowered to 45° C., 10 mL of anaerobic bacteria was added to the mixed slurry, and the stirring was continued to prepare a mixed slu...

Embodiment 3

[0056] (1) Prepare curing agent: add boric acid to 500mL distilled water and dissolve to obtain a saturated boric acid solution, weigh 20g CaCl 2 , 2g talcum powder, join in the saturated boric acid solution, make curing agent.

[0057] (2) Preparation of growth factor solution: 20.0 mg each of vitamin B1, vitamin B6, and nicotinic acid, 3 g of glucose, and 3 mL of 25% ethanol solution were added to 500 mL of tap water to prepare a growth factor solution.

[0058] (3) Preparation of immobilized anaerobic bacteria granules: 10 g of sodium alginate was added to 200 mL of growth factor solution, heated to 70° C., and continuously stirred at 60 rpm for 100 min to prepare a mixed slurry. After the temperature of the mixed slurry was lowered to 35° C., 10 mL of anaerobic bacteria was added to the mixed slurry, and the stirring was continued to prepare a mixed slurry containing anaerobic bacteria. Use a 50mL syringe with a needle to draw the mixed slurry containing anaerobic bacteri...

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Abstract

The embodiment of the invention discloses an anaerobic bacterium immobilization method. The method includes the following steps of firstly, adding an embedding material to a growth factor solution, heating the solution to 50-120 DEG C, and evenly stirring the solution to prepare mixed size, wherein the embedding material is at least one of sodium alginate and polyvinyl alcohol; secondly, adding anaerobic bacteria to the mixed size after the temperature of the mixed size is cooled to 30-45 DEG C, and stirring the size to prepare mixed size containing anaerobic bacteria; thirdly, dropwise adding the mixed size containing anaerobic bacteria to a curing agent, and standing for 2-24 h at the temperature of 0-10 DEG C to obtain anaerobic bacterium embedding particles, wherein the curing agent is prepared from calcium chloride, talcum powder and a saturated boric acid solution; fourthly, flushing the anaerobic bacterium embedding particles with water or an NaCl aqueous solution to obtain cured anaerobic bacterium particles, wherein the mass percentage of NaCl in the NaCl aqueous solution is smaller than or equal to 0.9%. According to the method, talcum powder is added to the curing agent, and the problem that adhesion is likely to be caused in the anaerobic bacterium immobilization process is solved.

Description

technical field [0001] The invention relates to the technical field of wastewater treatment, in particular to a method for immobilizing anaerobic bacteria. Background technique [0002] With the rapid development of the economy and the continuous growth of the population, the discharge of urban domestic sewage and industrial wastewater is increasing year by year, and the situation of water environment pollution is very severe. At present, anaerobic biological treatment is a technology commonly used in urban domestic sewage and industrial wastewater treatment. [0003] Anaerobic biological treatment is an organic sewage treatment method that uses anaerobic bacteria to degrade macromolecular organic matter in sewage into low molecular compounds, and then converts them into methane and carbon dioxide. Anaerobic biological treatment has the advantages of high removal rate of organic matter, less energy consumption in the treatment process, less sludge output, and less demand fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10C12N11/08C02F3/34
CPCC02F3/34C12N11/08C12N11/10
Inventor 蒋玮乔丽丽乔瑞平俞彬王玉慧张伦梁陈广升李璐
Owner POTEN ENVIRONMENT GRP
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