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Method for preparing cello-oligosaccharide through directional two-stage hydrolysis of cellulose

A technology of cellooligosaccharides and cellulose, which is applied in the field of cellooligosaccharide preparation by directional two-stage hydrolysis of cellulose, can solve the problem of low yield of cellooligosaccharides, achieve high resource utilization, simple method, and increase production output

Inactive Publication Date: 2016-04-20
JIANGSU KANGWEI BIOLOGIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art, and provide a method for preparing cellooligosaccharides by directional two-stage hydrolysis of cellulose. After dividing the β-glucosidase in the cellulase system, the yield of cellooligosaccharides is still not high.

Method used

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  • Method for preparing cello-oligosaccharide through directional two-stage hydrolysis of cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The hydrolysis time in the first hydrolysis treatment was 6 hours, and the hydrolysis time in the second hydrolysis treatment was 18 hours.

Embodiment 2

[0043] The hydrolysis time in the first hydrolysis treatment was 10 hours, and the hydrolysis time in the second hydrolysis treatment was 14 hours.

Embodiment 3

[0045] The hydrolysis time in the first hydrolysis treatment was 12 hours, and the hydrolysis time in the second hydrolysis treatment was 12 hours.

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Abstract

The invention belongs to the technical field of cello-oligosaccharide preparation, and discloses a method for preparing cello-oligosaccharide through directional two-stage hydrolysis of cellulose. The method includes the following steps of reaction stock solution preparation, temperature and pH value setting, substrate in-situ adsorption, solid-liquid separation treatment, first-time hydrolysis treatment, second-time hydrolysis treatment and cello-oligosaccharide purification. According to the method for preparing cello-oligosaccharide through directional two-stage hydrolysis of cellulose, the steps are simple and reasonable, a liquid phase containing cello-oligosaccharide is obtained by hydrolyzing xylo-oligosaccharide production residues through cellulase containing low beta-glucosidase, cello-oligosaccharide is obtained through solid-liquid separation and purification, the feedback inhibition of the product on the enzyme in the hydrolysis process can be effectively relieved by adopting the two-stage hydrolysis mode for hydrolysis, and the production yield of cello-oligosaccharide is greatly increased as the cello-oligosaccharide yield is higher than that in a non-staged hydrolysis mode. The method is simple, low in cost, high in resource utilization rate and capable of achieving industrial production of cello-oligosaccharide.

Description

【Technical field】 [0001] The invention relates to the technical field of cellooligosaccharide preparation, in particular to a method for preparing cellooligosaccharide by directional two-stage hydrolysis of cellulose. 【Background technique】 [0002] Fiber oligosaccharides can be obtained by hydrolysis of cellulose. Cellulose oligosaccharides have a certain solubility in water, but are slightly soluble or insoluble in organic solvents. Cellooligosaccharides, also known as cellooligosaccharides, are functional oligosaccharides that can promote mineral absorption. , Bifidobacterium proliferation factor, lower serum cholesterol, enhance body immunity, resist tumors and other unique physiological functions. It is an oligosaccharide with special physiological functions in humans, animals, plants, etc. It can be widely used in food and feed industries. [0003] Cellulase is a general term for a class of multicomponent enzymes that can hydrolyze β-1,4-glucosidic bonds of cellulose t...

Claims

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Application Information

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IPC IPC(8): C12P19/14
CPCC12P19/14
Inventor 朱汉静朱汉生刘镏陈冬劲
Owner JIANGSU KANGWEI BIOLOGIC CO LTD
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