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A kind of lymphokine tal and its coding gene and application

A technology of lymphokines and genes, applied in the direction of cytokines/lymphokines/interferons, applications, genetic engineering, etc., can solve problems that restrict the healthy and sustainable development of oyster farming in Hong Kong, the death of Hong Kong oysters, and the destruction of the breeding environment, and achieve The effect of promoting the proliferation of blood lymphocytes

Active Publication Date: 2019-03-08
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, with the continuous expansion of the breeding scale, the destruction of the breeding environment, and the frequent occurrence of diseases, the oysters in Hong Kong have died on a large scale, which seriously restricts the healthy and sustainable development of the oyster farming industry in Hong Kong.

Method used

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  • A kind of lymphokine tal and its coding gene and application
  • A kind of lymphokine tal and its coding gene and application
  • A kind of lymphokine tal and its coding gene and application

Examples

Experimental program
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Effect test

Embodiment 1

[0018] 1. RNA extraction

[0019] Hong Kong oyster total RNA was extracted using Trizol (Invitorgen), and the specific steps were as follows: (1) Take 50 mg of Hong Kong oyster tissue in a liquid nitrogen pre-cooled mortar, grind the tissue into powder, and transfer it to a centrifuge containing 1 ml Trizol. (2) Add 200 μL of chloroform, shake vigorously for 40 seconds, and place at room temperature for 5 minutes; (3) Centrifuge at 12,000×g for 10 minutes at 4°C, transfer the supernatant to a new tube; (4) Add 0.5ml of Isopropanol, mix well, precipitate overnight at -80°C; (5) centrifuge at 4°C, 12,000×g for 10 min, discard the supernatant; (6) wash the precipitate twice with 75% ethanol; (7) discard the supernatant, add 50 μL DEPC Water dissolves RNA.

[0020] 2. cDNA library construction

[0021] Full-length cDNA library construction using GeneRacer TM cDNA library construction Kit (Invitrogen), in which the specific steps of 5'RACE are as follows: (1) Calf intestinal ph...

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Abstract

The invention discloses a lymphokine TAL and a coding gene and application thereof. The lymphokine TAL is separated from crassostrea hongkongensis for the first time, the full length of the coding gene of the lymphokine TAL is 660 bp, and 219 amino acids are coded. It is found through functional analysis and identification that the lymphokine TAL of crassostrea hongkongensis can promote blood lymphocyte proliferation and is a novel lymphocyte growth factor. The lymphokine TAL has a significant effect of promoting blood lymphocyte proliferation and provides important theoretical and practical bases for disease prevention and treatment and disease resistance breeding of shellfish.

Description

technical field [0001] The invention belongs to the technical field of applied marine organisms, and in particular relates to a lymphokine TAL and its encoding gene and application. Background technique [0002] Hong Kong oysters (Crassostrea hongkongensis) are mainly distributed in numerous estuaries along the coast of South my country. They are also the most widely cultivated shellfish along the coast of Guangdong Province. They have the characteristics of large size, delicious taste, rich nutrition, and food tonic function, and are deeply loved by consumers. The annual aquaculture output has reached more than 700,000 tons, and the annual output value is about 2.5-3 billion yuan. It is one of the pillar industries of marine aquaculture in the coastal areas of South China. However, in recent years, with the continuous expansion of the breeding scale, the destruction of the breeding environment, and the frequent occurrence of diseases, the oysters in Hong Kong have died on a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/52C12N15/19C12N15/70C12N1/21A61K38/19A61P37/02C12R1/19
CPCA61K38/00C07K14/52
Inventor 李军张扬喻子牛刘映张跃环
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI