Anti-angiostatin receptor monoclonal antibody conjugated nanomicelle and its preparation and application

A monoclonal antibody and nano-micelle technology, applied in the field of biomedicine, can solve the problems of side effects, inability to distinguish cancer cells from normal cells, and toxicity, and achieve the effects of small side effects, superior anti-tumor activity, and reduced side effects

Active Publication Date: 2018-10-19
上海蓝西实验设备有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most clinical anticancer agents still cannot distinguish cancer cells from normal cells, resulting in systemic toxicity and serious side effects

Method used

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  • Anti-angiostatin receptor monoclonal antibody conjugated nanomicelle and its preparation and application

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Experimental program
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Embodiment 1

[0028] 1. Two-dimensional liquid chromatography separation technology to obtain protein HAI-178:

[0029] 2. High-efficiency and high-throughput fully human antibody technology to obtain high-expression engineering cell lines and prepare anti-HAI-178 specific humanized antibodies:

[0030] 3. Structural modification of polyoxyethylene-polyoxypropylene-polyoxyethylene block polymers:

[0031] a) Synthesis and characterization of carbonylated block polymer P123 (CT-P123);

[0032] The specific steps of polymer surface modification are: under the action of sodium hydroxide, the addition reaction of 2-butenenitrile and polyoxyethylene-polyoxypropylene-polyoxyethylene block polymer occurs, and then the addition reaction is carried out by stirring reaction The nitrile group contained in the product is hydrolyzed, and xylene is used to extract from the hydrolysis reaction product liquid to obtain a carboxyl PEO-PPO-PEO block copolymer product.

[0033] b) Linkage of CT-P123 and ant...

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Abstract

The invention discloses a combined nano-micelle of monoclonal antibodies for resisting vascular inhibition receptors. The nano-micelle is formed by connecting the monoclonal antibodies for resisting vascular inhibition receptors with carbonylation polyoxyethylene-polyoxypropylene-polyoxyethylene block polymers. The invention further discloses the immune micelle prepared by wrapping the monoclonal antibodies for resisting vascular inhibition receptors as medicine carriers on taxol, and preparation and application of the immune micelle. Humanized antibodies contained in the nano-micelle are low in immunogenicity and small in side effect; meanwhile, the immune micelle is stable in wrapping performance, high in wrapping rate and good in antibody activity.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an anti-angiostatin receptor monoclonal antibody combined with nano micelles and its preparation and application. Background technique [0002] Tumor is one of the three major diseases that seriously threaten human health today. Angiostatin receptor (AngiostatinReceptor F1F0-ATP synthase) plays an important role in ATP synthesis in tumor cells. In normal human tissues, angiostatin receptors are mainly located on the inner membrane of mitochondria, but a small amount is also present on the cell membrane of endothelial cells. In contrast, many tumor cells overexpress angiostatin receptors, and a large number of them are located on the cell membrane. Therefore, the angiostatin receptor on the tumor cell membrane is a potential antitumor drug target. [0003] As one of the fastest growing and most profitable markets in the pharmaceutical industry, the monoclonal antibody market has achi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/42A61K31/337A61P35/00
Inventor 倪健
Owner 上海蓝西实验设备有限公司
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