Time-resolved fluorescent immunoassay kit for detecting glyphosate and detecting method of kit

Abstract

The invention discloses a time-resolved fluorescent immunoassay kit for detecting glyphosate and a detecting method of the kit. The kit is composed of a porous coating plate, a buffer solution, a glyphosate standard product, a glyphosate antibody freeze-dried product, a europium-labeled goat-anti-mouse antibody, a washing solution and an enhancing solution. The method includes the following steps of firstly, preparing immunogen; secondly, preparing coating antigens; thirdly, preparing monoclonal antibodies; fourthly, preprocessing and detecting a sample. The kit is short in detecting time, high in average recovery rate, simple in sample preprocessing, capable of conducting site operation and detection, wide in application range and low in detection cost, and meanwhile has the advantages of being high in detection specificity, small in inside-batch and inter-batch difference, high in sensitivity, easy and rapid to operate, particularly suitable for detecting a large batch of samples and the like.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a time-resolved fluorescent immunoassay kit for detecting glyphosate and a detection method thereof. Background technique [0002] With the continuous development of agricultural science and technology, many pharmaceutical factories in my country can produce high-efficiency, broad-spectrum, and low-toxic herbicides, and glyphosate is one of them. Glyphosate is a systemic conduction type broad-spectrum killing glyphosate, mainly by inhibiting enolpyruvyl shikimelin phosphate synthase in plants, thereby inhibiting the conversion of shikime to phenylalanine, tyrosine and tryptophan. transformation, the protein synthesis is disturbed and the plant dies. [0003] Glyphosate is known for its strong systemic conductivity. It can not only be transmitted to the underground part through the stems and leaves, but also can be transmitted between different tillers of the same ...

AI Technical Summary

Problems solved by technology

[0004] Glyphosate residue analysis generally uses gas chromatography (GC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC / MS). These methods are sensitive and accurate, and can simultaneously determine multiple drugs, but require Expensive instruments, complex, tedious and time-consuming sample pretreatment, high testing costs, and professional operation are required, making it difficult to meet the needs of on-site, batch, and rapid testing of samples

There are currently no patents or literature reports on a time-based fluorescent immunoassay for glyphosate detection