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A kind of preparation method of toad venom extract and sustained-release pellets

A technology of sustained-release pellets and extracts, which can be used in pharmaceutical formulations, drug combinations, drug delivery, etc., and can solve problems such as treating the symptoms but not the root cause, ineffective reduction of malignant tumors, and poor analgesic effects

Active Publication Date: 2019-10-25
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional Chinese medicine has certain advantages in the treatment of malignant tumors. It can enhance human immunity, inhibit the growth of malignant tumors, and avoid the side effects of radiation and chemotherapy on normal human body cells. At present, there are some traditional Chinese medicines for the treatment of malignant tumors on the market, but these Most traditional Chinese medicines for the treatment of malignant tumors have weak lethality to malignant tumor cells, resulting in insignificant shrinkage of malignant tumors, poor analgesic effect, inaccurate curative effect, and treating symptoms but not the root cause

Method used

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  • A kind of preparation method of toad venom extract and sustained-release pellets
  • A kind of preparation method of toad venom extract and sustained-release pellets
  • A kind of preparation method of toad venom extract and sustained-release pellets

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Investigation of the extraction process of bufolides

[0038] The particle size of the medicinal material, the amount of solvent used, the number of extractions, the extraction time and the concentration of ethanol are the main factors affecting the extraction effect. The small particle size of the medicinal material is conducive to the penetration of the solvent into the interior of the medicinal material particles, which is beneficial to the extraction of active ingredients, but the crushing of the medicinal materials is too fine, which is not conducive to the dissolution of the active ingredients. According to the solubility of bufolides in ethanol, most of them are extracted with ethanol, so ethanol is selected as the extraction solvent in this part. good extraction process.

[0039] 1.1 Orthogonal design experiment

[0040] Taking the total amount of tobugenin and cinobufaginin-total toxin base as an index, the amount of solvent, extraction time, et...

Embodiment 2

[0052] Embodiment 2: the investigation of indole alkaloid extraction process

[0053] 1.1 Orthogonal design experiment

[0054] Taking 5-hydroxytryptamine as an index, the amount of water, extraction time, and extraction times were investigated to obtain the optimal extraction process. Use L 9 (3 4 ) orthogonal design table for the experiment, respectively weighed 9 portions of toad venom fine powder, 0.5 g in each portion, extracted according to the extraction process orthogonal design table, and each extracted solution was filtered and combined for subsequent use. The content of the water-soluble matter of toad venom was determined by the above-mentioned ultraviolet spectrophotometry method of water-soluble matter. See Table 2-1 and Table 2-2.

[0055] Table 2-1. Water-soluble component extraction factor level table

[0056]

[0057] Table 2-2. Orthogonal test result table L 9 (3 4 )

[0058]

[0059] Table 2-3. Analysis of Variance

[0060]

[0061] From t...

Embodiment 3

[0066] Example 3: In vitro MTT method for screening anti-tumor components of toadstool

[0067] The chemical name of MTT is 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide, which is a yellow dye. The MTT assay is a method to measure cell survival and growth. Detection principle: The succinate dehydrogenase present in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystalline formazan (Formazan) and deposit in the cells. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its optical absorption (OD) value is measured at a wavelength of 490nm with a microplate reader to reflect cell survival. This method has high sensitivity and strong specificity, and has been used as one of the routine methods for in vitro drug screening of tumor patients. This part of the experiment is mainly to screen out the anti-tumor active ingredients of Chansu by MTT method, which will lay the foundation for future researchers to study the...

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Abstract

The invention discloses a venenum bufonis extractive and a preparation method of sustained-release pellets of the venenum bufonis extractive, and belongs to the technical field of pharmacies. The venenum bufonis extractive comprises the following technological steps that venenum bufonis powder is added into 60-90% ethyl alcohol with the amount 60-100 times that of the powder, extraction is performed for 1-3 times and performed for 60-120 min every time, and effective ingredients of bufogenin are obtained; 40-60-fold water is added into dregs, extraction is performed for 1-3 times and performed for 30-60 min every time, and effective ingredients of indole alkaloid are obtained; the effective ingredients are mixed for concentration drying, and the venenum bufonis extractive is obtained. On the one hand, the effective ingredients of bufogenin and indole alkaloid can be effectively extracted; on the other hand, working basis is provided for the screening of the effective ingredients of the venenum bufonis, preparation technology and dosage form research and novel drug research, and conditions are created for improving the material basis of the effective ingredients of the novel traditional Chinese medicine and controlling the quality of the effective ingredients.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a preparation method of toad venom extract and sustained-release pellets thereof. Background technique [0002] Throughout the ages, human beings have been making unremitting efforts to treat cancer, but malignant tumors are still on the rise, threatening human health constantly. According to the latest data, in the next 20 years, the number of new cancer patients worldwide will increase from 10 million to 50 million per year, and the number of cancer deaths will increase from 6 million to 10 million per year. There are many factors that induce malignant tumors. The fundamental reason is that the body’s immune system is weak and cannot accurately detect cancer cells and inhibit the survival of cancer cells in time, causing cancer cells to aggregate, fission and proliferate. Knot. "Righteous Qi" is the body's ability to resist disease caused by pathogenic factors. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/65A61K9/16A61K9/58A61P35/00
Inventor 黄绳武黄挺
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY