A kind of method of producing selenium-enriched Cordyceps sinensis mycelia raw material
A technology of cordyceps sinensis mycelium and selenium enrichment is applied in the field of producing selenium-enriched cordyceps sinensis mycelium raw materials to achieve the effects of protecting public health and reducing production costs
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Embodiment 1
[0071] Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.6g / 100mL medium, the amount of bran used is 4.8g / 100mL medium, and KH 2 PO 4 0.27g / 100mL, MgSO 4 ·7H 2 O 0.27g / 100mL, cysteine hydrochloride 50mg / 100mL, sodium copper chlorophyllin 10mg / 100mL, sodium selenite 1.33mg / 100mL, natural pH, add water to the required volume, sterilize at 121°C for 30min, Used as a culture medium for fermentation, the sample volume of the shake flask was 40%, the inoculum volume was 10%, and the culture temperature was 20 o C, rotating speed 180r / min, add 1.33mg / 100mL sterilized sodium selenite solution after fermentation 2d, total fermentation time is 5d. The following steps (3) to (10) in the technical solution described in the foregoing summary of the invention are implemented. The results are: mycelium dry weight 4.55 g / 100mL medium, exopolysaccharide 218.5mg / 100mL medium, mycelia polysaccharide 233.4mg / 100mL medium, c...
Embodiment 2
[0073] Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.0g / 100mL medium, the amount of bran used is 3.0g / 100mL medium, and KH 2 PO 4 0.015g / 100mL, MgSO 4 ·7H 2 O 0.015g / 100mL, cysteine hydrochloride 20mg / 100mL, sodium copper chlorophyllin 5mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use as medium for fermentation, shake bottle The sample volume was 40%, the inoculum volume was 10%, and the culture temperature was 23 o C, rotating speed 180r / min, add 0.21mg / 100mL sterilized sodium selenite solution after 2 days of fermentation, the total fermentation time is 5 days. Culture time 5d. The following steps (3) to (10) in the technical solution described in the foregoing summary of the invention are implemented. The results were: mycelium dry weight 2.95g / 100mL medium, exopolysaccharide 139.8mg / 100mL medium, mycelia polysaccharide 145.6mg / 100mL medium, cordycepin con...
Embodiment 3
[0075] Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.6g / 100mL medium, the amount of bran used is 3.6g / 100mL medium, and KH 2 PO 4 0.14g / 100mL, MgSO 4 ·7H 2 O 0.14 g / 100mL, cysteine hydrochloride 35mg / 100mL, sodium copper chlorophyllin 7.5mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use as medium for fermentation, shake The sample volume in the bottle is 40%, the inoculum volume is 10%, and the culture temperature is 20 o C, rotating speed 180r / min, add 0.77mg / 100mL sterilized sodium selenite solution after 2 days of fermentation, the total fermentation time is 6 days. The following steps (3) to (10) in the technical solution described in the foregoing summary of the invention are implemented. The results were: mycelium dry weight 3.88g / 100mL medium, exopolysaccharide 186.4mg / 100mL medium, mycelia polysaccharide 196.5mg / 100mL medium, cordycepin content 1.62mg / g...
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