Femtosecond time resolution multi-channel lock-phase fluorescence spectrophotometer based on optical parametric amplification

Abstract

The invention provides a femtosecond time resolution multi-channel lock-phase fluorescence spectrophotometer based on optical parametric amplification and relates to the technical field of spectral measurement. With the adoption of the spectrophotometer, output laser of a laser source is divided into two beams through a beam splitting sheet; one beam of the laser is emitted into a sample fluorescence light generation and collection system and is used for generating stimulating light for stimulating sample fluorescence light; the other beam of the laser is emitted into a pumping laser generation system and is used for generating pumping light for amplifying fluorescence light; the fluorescence light amplification can be carried out in an optical parametric amplification light path; and amplified fluorescence light is transmitted to a data acquisition system connected with the spectrophotometer through a light outlet of the spectrophotometer and then is subjected to time resolution fluorescence detection, and the data acquisition system is a multi-channel photodiode array interface lock-phase amplification data acquisition system. The spectrophotometer provided by the invention has the advantages that the light path is simple to adjust, the detection sensitivity is high, a measured result is relatively accurate and a spectrum is not easy to distort.

Description

technical field [0001] The invention relates to the technical field of spectrum measurement, in particular to a femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parameter amplification. Background technique [0002] Femtosecond time-resolved fluorescence spectroscopy is an important research method in the fields of modern photophysics, photochemistry, and light energy absorption and storage in biological systems. Unlike femtosecond time-resolved transient absorption systems, time-resolved fluorescence directly reflects ultrafast processes such as energy transfer and electron transfer in excited states. The current common time-resolved fluorescence detection methods include streak camera technology, optical Kerman technology, fluorescence up-conversion technology and optical parametric fluorescence amplification technology. Among these methods, the optical parametric fluorescence amplification technology has the advantages of hi...

AI Technical Summary

Problems solved by technology

[0005] 1. The size of the optical fiber is limited (diameter of a single fiber: 400 microns), which makes the adjustment of the optical path more troublesome. The signal to be detected must be accurately adjusted to a specific height before entering the spectrometer, and the light spot must be precisely focused on the incident slit to get a stronger signal. signal of;

[0006] 2. Due to the existence of chromatic aberration, the light in the red area and the blue area cannot be focused at the rear focal plane of the spectrometer at the same time, resulting in the difference in fiber coupling efficiency and finally resulting in spectral shape distortion;

[0007] 3. The transmission loss of the optical fiber loses part of the energy, which reduces the detection sensitivity;

[0008] 4. The light transmission range of the optical fiber limits the spectral range of the instrument, and it is difficult to extend the instrument to the ultraviolet and infrared bands;

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