A process for preparing high-purity gardenia blue pigment of different hues

A high-purity gardenia blue pigment technology, which is applied in the field of preparing high-purity gardenia blue pigments with different hues, can solve the problems of low product quality and long reaction time

Active Publication Date: 2018-09-18
HENAN ZHONGDA HENGYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the problems of long reaction time and low product quality in the existing process of extracting gardenia blue pigment, the present invention provides a process for preparing high-purity gardenia blue pigment with different hues

Method used

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  • A process for preparing high-purity gardenia blue pigment of different hues

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] see figure 1 , the process is as follows:

[0027] 1) Hydrolysis reaction. Take 200g of geniposide (content 80%) into the reactor, add 414g of water, stir to completely dissolve the geniposide in the water, adjust the pH to 4.5 with dilute hydrochloric acid, and heat the solution to 50°C in a 50°C water bath while stirring. ℃; then add 6.87g β-glucosidase, react for 4.5 hours, adjust the pH to 8.0 by adding NaOH;

[0028] 2) Polymerization. Feed compressed air into the reaction kettle with a flow rate of 50L / min; increase the temperature of the water bath to 70°C, add 24.7g of sodium glutamate at the same time, and carry out a color reaction. During the reaction, carry out moderate water replenishment. After 2 hours of reaction, stop ventilation ; The experimental data are shown in Table 1.

[0029] The experimental data in the table 1 embodiment 1

[0030] Response time / hour

R1

R2

Compressed air flow / L / min

1

2.61

2.77

50

2 ...

Embodiment 2

[0034] see figure 1 , the process is as follows:

[0035] 1) Hydrolysis reaction. Take 200g of geniposide (content 80%) into the reaction kettle, add 410g of water, stir to completely dissolve the geniposide in the water, adjust the pH to 4.3 with dilute hydrochloric acid, and heat the solution to 50°C in a 50°C water bath while stirring. ℃; then add 6.87g β-glucosidase, react for 4.5 hours, adjust the pH to 8.1 by adding NaOH;

[0036] 2) Polymerization. Introduce compressed air into the reaction kettle with a flow rate of 50 L / min, increase the temperature of the water bath to 70°C, and add 24.7 g of sodium glutamate at the same time to carry out a color reaction. The reaction lasts for 5 hours. The experimental data are shown in Table 2.

[0037] The experimental data in the table 2 embodiment 2

[0038] Response time / hour

R1

R2

compressed air flow

1

2.67

2.81

50

2

3.80

3.08

50

3

4.21

2.92

50

4

3.85...

Embodiment 3

[0042] see figure 1 , the process is as follows:

[0043] 1) Hydrolysis reaction. Take 900g of geniposide (content 80%) into the reaction kettle, add 9540g of water, stir to completely dissolve the geniposide in the water, adjust the pH to 4.4 with dilute hydrochloric acid, heat it to 50°C in a water bath, and keep it at a constant temperature; then add 114g of β-fiber Sulfase, stirring for hydrolysis reaction for 5 hours, after the reaction, adjust the pH to 8.2 with NaOH solution;

[0044] 2) Polymerization. Add 315g of sodium glutamate and 100g of phenylalanine, and at the same time pass compressed air at a flow rate of 200L / min; after 1 hour of reaction, the ventilation rate is reduced to 100L / min; after 2 hours of reaction, stop the ventilation; after 2.5 hours of reaction Afterwards, cool down with condensing circulating water, stop reaction; Obtain the gardenia blue reaction solution 11.42kg of R1=3.95, R2=2.79, E600=8.98;

[0045] 3) Through refining, you can get: ...

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Abstract

The invention discloses a process for preparing high-purity gardenia blue pigment in different hues. According to the process, gardenoside is used as the raw material and hydrolyzed with beta-glucosidase to obtain genipin, a colored product is generated through polymerization reaction of genipin and amino acid (primary amino compounds) under the action of an oxidizing agent, and the high-purity gardenia blue pigment is obtained through separation and purification. The process comprises the steps of hydrolysis reaction, polymerization reaction, separation and purification, dehydration forming and checking and verification. By the adoption of the process, the gardenia blue preparing cycle can be shortened to 2.5-5 h, the maximum absorption wavelength of the prepared gardenia blue pigment reaches 600 nm, and the hue of the gardenia blue pigment can be regulated as needed.

Description

technical field [0001] The invention belongs to the technical field of plant pigment extraction, and in particular relates to a process for preparing high-purity gardenia blue pigments with different hues. Background technique [0002] Gardenia blue pigment is a natural water-soluble blue pigment that has received wide attention in recent years. It uses the chemical component geniposide contained in the Rubiaceae plant—Gardenia jasminoides as raw material, uses β-1,4-glucosidase to hydrolyze geniposide, removes the glucose group at the end of its molecule, and obtains geniposide Genipin undergoes a series of polymerization and rearrangement reactions with amino acids. After the reaction is completed, the gardenia blue pigment can be obtained by drying. [0003] At present, gardenia blue pigment is mainly fermented by microorganisms with high production of β-1,4-glucosidase, and then macroporous resin is generally used to desalt, refine and purify the fermentation broth. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/00C09B61/00
CPCC09B61/00C12P1/00
Inventor 田浩潘天义文雁君李林正章文晋焦军伟
Owner HENAN ZHONGDA HENGYUAN BIOTECH CO LTD
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