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Bioelectrochemical detection method for vitamin B2

A bioelectrochemical and detection method technology, applied in the direction of material electrochemical variables, can solve the problems of poor data repeatability, high detection cost, and complicated operation process, and achieve the effect of strong specificity, low cost, and high sensitivity

Active Publication Date: 2016-06-01
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The object of the present invention is to provide a new specificity assay VB 2 The method solves the problems of high detection cost, expensive detection equipment, poor data repeatability, and complicated operation process (including sample preparation and sample pretreatment) existing in the prior art.

Method used

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  • Bioelectrochemical detection method for vitamin B2
  • Bioelectrochemical detection method for vitamin B2
  • Bioelectrochemical detection method for vitamin B2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Strain used: Shewanella oneidensis, purchased from ATCC American Type Culture Collection, strain number ATCC700550.

[0044] (2) Obtaining Shewanella bacteria liquid: Inject Shewanella bacteria into 20mL LB medium (containing yeast extract 5g / L, tryptone 10g / L and sodium chloride 5g / L, pH=7.0) Bacterial strains were cultured at a temperature of 30° C. and a shaking speed of 200 rpm for 16 hours to obtain a bacterial liquid.

[0045] (3) Prepare reaction buffer for bioelectrochemical sensors: mix 0.6mL LB liquid medium (containing tryptone 10g / L, yeast extract 5g / L and sodium chloride 5g / L, pH=7.0) and 11.4mL LM9 medium (with Na 2 HPO 4 .12H 2 O17.8g / L, KH 2 PO 4 g / L, NaCl0.5g / L, NH 4 Cl1g / L) and mix thoroughly, and additionally add sodium lactate, calcium chloride, and magnesium sulfate to make the final concentrations reach 18mM, 0.1mM, and 1mM, respectively.

[0046] (4) Assembly of the bioelectrochemical sensor: a three-electrode working method was adopted...

Embodiment 2

[0057] It is basically the same as Example 1, but with the following changes: the working electrode is carbon felt. The test chart obtained is attached Figure 5 shown. Working electrode using carbon felt can also be done for VB 2 For detection, the working electrode is not limited to carbon cloth.

Embodiment 3

[0059] It is basically the same as Example 1, but with the following changes: the applied voltage of the working electrode is -0.2 volts. Current signal such as Figure 6 shown. The applied voltage of the working electrode is -0.2 volts, which can also detect VB 2。

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Abstract

The invention belongs to the technical field of biochemical detection, and particularly relates to a bioelectrochemical detection method for vitamin B2. The detection method is characterized by comprising the following steps: forming a reaction liquid system by using an electron acceptor of Shewanella oneidensis, Shewanella oneidensis liquid and a pH buffer solution together; controlling a certain electric potential by adopting a three-electrode system; adding a VB2-containing sample into a reaction liquid of a bioelectrochemical sensor, and detecting the change of a current value; determining the concentration of the VB2 in the sample according to a linear relation between the change of the concentration of the VB2 and a current response value; the detection method does not depend on large-scale instruments and equipment, is low in detection cost, simple in operation and high in accuracy and specificity, and can be applied to detecting the VB2 in biochemical samples, food and medicines.

Description

technical field [0001] The invention relates to a vitamin B 2 The bioelectrochemical detection method belongs to the technical field of biochemical detection. Background technique [0002] Vitamin B 2 , VB 2 , that is riboflavin, the chemical formula is C 17 h 20 N 4 o 6。 It is a water-soluble B vitamin, which plays an important role in the energy conversion process of carbohydrates, proteins and fats in the body, and is of great significance to human health. [0003] VB 2 Deficiency can cause metabolic disorders in the human body, which can lead to various diseases. The lesions are mostly manifested as inflammation of the mouth, eyes, and external genitalia, such as angular cheilitis, cheilitis, glossitis, conjunctivitis, and scrotum. It is worth noting that the human body cannot synthesize VB itself 2 , can only be obtained through diet. Therefore, VB 2 Content is an important indicator for evaluating food nutrition and its functionality. At the same time, due...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 雍阳春司荣炜翟丹丹
Owner JIANGSU UNIV
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