Method for extracting water-soluble dietary fibers from honeysuckle stems

A technology of dietary fiber and honeysuckle, applied in food extraction, function of food ingredients, food science, etc., can solve the problem of low utilization rate of honeysuckle, achieve the effect of improving dietary fiber structure, good extraction rate, and increasing utilization rate

Active Publication Date: 2016-06-08
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the defects in the prior art, the present invention aims to provide a method for extracting water-soluble dietary fiber f

Method used

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  • Method for extracting water-soluble dietary fibers from honeysuckle stems
  • Method for extracting water-soluble dietary fibers from honeysuckle stems
  • Method for extracting water-soluble dietary fibers from honeysuckle stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Wash and drain the honeysuckle vines, dry them in a constant temperature drying oven, crush them, and pass through a 40-mesh sieve [4] .

[0048] Accurately weigh 3.000g of 12 pretreated honeysuckle samples and place them in dry and clean conical flasks, add water to disperse, and set the mass ratio of solid to liquid as 1:9, 1:12, 1:15 and 1:17 respectively , each material-liquid ratio was parallelized three times, adjusted pH 6.0 with hydrochloric acid and sodium hydroxide, added 3-5 drops of buffer solution, added α-amylase with a pipette gun, sealed, and enzymatically hydrolyzed for 45 minutes in a constant temperature water bath at 60°C ;Inactivate the enzyme by raising the temperature, adjust the pH to 4.5 with hydrochloric acid after cooling to room temperature, add buffer solution, add glucoamylase, re-seal, enzymatically hydrolyze for 1 hour at a constant temperature of 45°C, and inactivate the enzyme by heating [3]. After cooling to room temperature, adjust ...

Embodiment 2

[0060] Wash and drain the honeysuckle vines, dry them in a constant temperature drying oven, crush them, and pass through a 60-mesh sieve.

[0061] Accurately weigh 3.000g of 12 pretreated honeysuckle samples and place them in dry and clean conical flasks, add water to disperse, the mass ratio of solid to liquid is 1:12, adjust the pH to 6.0 with hydrochloric acid and sodium hydroxide, add 3- 5 drops of buffer solution, add α-amylase with a pipette gun, seal, and enzymolyze in a constant temperature water bath at 60°C for 45 minutes; heat up to inactivate the enzyme, cool to room temperature, adjust the pH to 4.5 with hydrochloric acid and sodium hydroxide, and add buffer solution , add glucoamylase, re-seal, enzymatically hydrolyze at 45°C for 1 hour, heat up to inactivate the enzyme [4] . After cooling to room temperature, adjust Ph4.8, add a buffer solution, add respectively a concentration of 1.0% (ie 0.01g / ml) cellulase 40ul, a concentration of 0.3% (ie 0.003g / ml) xylana...

Embodiment 3

[0066] Wash and drain the honeysuckle vines, dry them in a constant temperature drying oven, crush them, and pass through a 40-mesh sieve.

[0067] Accurately weigh 3.000 g of 15 pretreated honeysuckle samples and place them in dry and clean conical flasks, add water to disperse, the mass ratio of solid to liquid is 1:12, adjust the pH to 6.0 with hydrochloric acid and sodium hydroxide, add 3- 5 drops of buffer solution, add α-amylase with a pipette gun, seal, and enzymolyze in a constant temperature water bath at 60°C for 45 minutes; heat up to inactivate the enzyme, cool to room temperature and adjust the pH to 4.5 with hydrochloric acid, add buffer solution, and add glucoamylase , re-sealed, enzymatically hydrolyzed for 1 hour at a constant temperature of 45°C, and inactivated by heating [4] . After cooling to room temperature, adjust Ph4.8, add buffer solution, and add 40ul concentrations of 0.5%, 0.8%, 1.0%, 1.2%, 1.5% respectively (0.005g / ml, 0.008g / ml, 0.010g / ml, 0.012...

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Abstract

The invention discloses a method for extracting water-soluble dietary fibers from honeysuckle stems, and belongs to the technical field of comprehensive utilization of plants. The method includes the steps that the honeysuckle stems serve as raw materials, and are dried, smashed and sieved, pH is adjusted, the processed honeysuckle stems are subjected to three times of enzymolysis, then subjected to enzyme deactivation and centrifuged, and filter liquid and sediment are obtained respectively; the filter liquid is concentrated, subjected to alcohol precipitation, centrifuged and dried to obtain the water-soluble dietary fibers. According to the method, the honeysuckle stems serve as raw materials, fine and deep processing of agricultural and sideline product raw materials is achieved, resource waste is reduced, and meanwhile a new path is provided for comprehensive utilization of honeysuckle stem products.

Description

technical field [0001] The invention relates to the technical field of plant comprehensive utilization, in particular to a method for extracting water-soluble dietary fiber from honeysuckle vines. Background technique [0002] The physiological role of dietary fiber in the human body has been gradually recognized by people. It has become an internationally popular health food like vitamins, and has been listed as the "seventh nutrient" after sugar, protein, fat, water, minerals and vitamins. ". Since it lowers blood lipids in the body [1] , reduce constipation, prevent hemorrhoids, intestinal sores, colon cancer [2] ,diabetes [2] , breast cancer [2] , obesity and other diseases, promote gastrointestinal motility, help digestion, and can chelate cholesterol, porphyrin and heavy metal toxic substances in the digestive tract [3] Therefore, it is regarded as a preventive factor for "diseases of civilization" (diabetes, fatty liver, etc.). In 1991, the World Health Organiza...

Claims

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Application Information

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IPC IPC(8): A23L33/24
CPCA23V2002/00A23V2200/238A23V2200/30A23V2250/5116A23V2300/14
Inventor 刘谦郭凤倩张永清蒲高斌李佳
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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