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Effective part of ginseng stems and leaves, as well as preparation method and application thereof

A technology of ginseng stems and leaves and effective parts, which is applied in the field of effective parts of ginseng stems and leaves and its preparation, can solve problems such as unclear interactions, high quality control costs, and complicated drug components, and achieve strong quality controllability and reduce Difficulty in separation and purification, and the effect of great promotion and application value

Active Publication Date: 2016-06-08
成都普瑞法科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prescription of this medicine is reasonable, has synergistic effect, and can be used in combination with medicines for the treatment of diabetic nephropathy. However, the composition of the medicine is complex, the interaction between the ingredients is not clear, the cost of quality control is high, the stability is poor, and the production cost is relatively high. high

Method used

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  • Effective part of ginseng stems and leaves, as well as preparation method and application thereof
  • Effective part of ginseng stems and leaves, as well as preparation method and application thereof
  • Effective part of ginseng stems and leaves, as well as preparation method and application thereof

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preparation example Construction

[0024] The preparation method of the effective part of ginseng stem and leaf of the present invention comprises the following steps:

[0025] a. Separation and purification: take the ginseng stem and leaf extract, put it on the pretreated chromatographic column, and then use pure water and lower alcohol solution to elute, collect the lower alcohol solution eluate, after concentration, place it at room temperature for crystallization, and obtain crystals;

[0026] b. Recrystallization: Take the crystals obtained in step a and recrystallize with a lower alcohol-water system until ginsenoside Rg 1 and the total mass fraction of Re is greater than 90%, preferably ginsenoside Rg 1 The total mass fraction of Re and Re is greater than or equal to 95%.

[0027] Wherein, the ginseng stem and leaf extract can be obtained by using a conventional commercially available extract, or by dissolving a commercially available ginseng stem and leaf extract, or by extracting ginseng stem and leaf...

Embodiment 1

[0040] (1) Take 20kg of cut ginseng stems and leaves (purchased from the medicinal material wholesale market), first rinse and soak with tap water for 20 minutes, then add pure water to reflux and heat to extract 3 times, and the amount of water added each time is 8, 6, 4 times, continue heating for 1 hour after boiling each time, and combine the extracts for 3 times.

[0041] (2) Get the ginseng stem and leaf extract obtained in step (1), centrifuge and filter, take the filtrate, and purify it on a pretreated nm200 polymer chromatographic column (methacrylate matrix, Suzhou Nanomicro Technology Co., Ltd.). Pure water, 30% ethanol elution, discard the tannin impurities eluted by pure water, collect rich ginsenoside Rg 1 / Re lower alcohol solution eluate, concentrated to a small amount, and left at room temperature for 24 hours to obtain crystals.

[0042] (3) Take the crystals obtained in step (2) and recrystallize with ethanol / water system to obtain the final effective fract...

Embodiment 2

[0044] (1) Get 15kg of cut ginseng stems and leaves (purchased from the medicinal materials wholesale market), first rinse and soak them with tap water for 20 minutes, then add 50% ethanol to reflux and heat to extract 3 times, and the amount of water added each time is 10 and 6 times the amount of medicinal materials respectively. , 6 times, continue to heat for 1 hour after heating and boiling each time, combine the extracts for 3 times, and concentrate under reduced pressure until alcohol-free;

[0045] (2) Take the ginseng stem and leaf concentrate obtained in step (1), centrifugally filter, take the filtrate, and purify on a pretreated PS100 polymer chromatographic column (styrene matrix, Suzhou Nanomicro Technology Co., Ltd.), followed by pure water , 25% methanol for elution, discard the tannin impurities eluted by pure water, and collect rich ginsenoside Rg 1 / Re lower alcohol solution eluate, concentrated to a small amount, and left at room temperature for 24 hours to...

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Abstract

The invention relates to an effective part of ginseng stems and leaves, as well as a preparation method and an application thereof, belongs to the technical field of natural medicinal materials, and aims to provide an effective part of ginseng stems and leaves having simple composition for treating or preventing diabetes mellitus and related complications. The active ingredient of the effective part comprises ginsenoside Re and ginsenoside Rg1 in a mass ratio of 1:(0.667-5). The effective part of ginseng stems and leaves has the characteristics of exact active ingredient, simple composition, strong quality controllability, good product stability, small dosage, better pharmacological activity and the like. The preparation method comprises the step of separating and purifying ginsenoside Re and ginsenoside Rg1 as a whole, and the effective part of the ginseng stems and leaves has high ingredient stability, strong quality controllability, simple preparation process and high preparation efficiency, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to effective parts of ginseng stems and leaves, a preparation method and application thereof, and belongs to the technical field of natural medicines. Background technique [0002] According to the latest statistics from IDF, in 2013, the global prevalence of diabetes among adults aged 20 to 79 was 8.3%, and the number of patients had reached 382 million, of which 80% were in middle- and low-income countries, and these countries showed a rapid increase the trend of. It is estimated that by 2035, nearly 592 million people worldwide will suffer from diabetes. Of the current population with diabetes, 175 million (46%) are undiagnosed. In 2013, the global prevalence of impaired glucose tolerance (IGT) was 6.9%, with a total of 316 million patients; this number is expected to increase to 471 million by 2035. In recent years, diabetes has shown trends such as younger age, higher growth rate, higher morbidity rate, and rising mortalit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P3/10C07J17/00
CPCA61K31/704C07J17/005A61K2300/00
Inventor 谢海峰杨金辉罗明峰郭建龙胡云岭
Owner 成都普瑞法科技开发有限公司
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