Preparation for inhibiting poxvirus infections
A pox virus and preparation technology, applied in the field of preparations for inhibiting pox virus infection, can solve the problems of epidemic spread, difficult prevention and control effects of specific prevention and treatment drugs, and no curative effect, and achieve the effect of inhibiting infection
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Embodiment 1
[0038] Embodiment 1, the preparation of plant extract compound formula virus infection inhibitor
[0039] Epigallocatechin gallate (EGCG), tannic acid and astragalus polysaccharide are uniformly mixed according to a mass ratio of 1:1:1.5 to obtain a plant extract compound formulation.
[0040] Before the experiment, it was dissolved in PBS buffer, made into 2560 μg / ml (the total concentration of EGCG, tannic acid and astragalus polysaccharide in the solution), filtered, and after sterilization, it was stored at -20°C for later use.
Embodiment 2
[0041] Embodiment 2, cytotoxicity test
[0042] In this example, the neutral red phagocytosis method was used to measure the cytotoxicity of the compound formulation prepared in Example 1 to mammalian cells. The specific operation is as follows:
[0043] The compound formulation preparation solution (2560 μg / ml) prepared in Example 1 was diluted step by step to obtain a total of 6 concentration. Then the dilutions of different dilutions were added to the 96-well cell culture plate with Vero cells and the cell density was about 80%, 100 μl in each well, and 4 duplicate holes were made for each dilution. Add compound formula preparation) as a control, after 2 hours of action, discard the test solution, add cell maintenance culture medium, add 200 μ l to each well, place it in a cell incubator and cultivate, after 48 hours, add 0.1% (0.1g / 100mL) to each well Neutral red 25μl, after reacting at 37°C for 1.5h, suck out the liquid in each well and rinse twice with PBS. Add 100 μ...
Embodiment 3
[0048] Embodiment 3, pox virus infection inhibition test
[0049] In this example, the CPE method was used to measure the inhibitory effect of the compound formulation prepared in Example 1 on poxvirus infection. The tested poxvirus was vaccinia virus WR strain.
[0050] Take a 96-well culture plate cultured with Vero cells that has grown into a single layer of cells with a growth density of about 80%, pour off the culture medium, rinse the cells with PBS for 3 times, and add them under the three conditions of A, B, and C respectively. The compound formula preparation that example 1 prepares:
[0051] A. Simultaneously with virus adsorption: put an equal volume of 2×100TCID 50 After mixing the poxvirus virus solution with 2 times the concentration of the test substance solution, add 100 μl / well of the mixed solution to the cell culture plate, place it in the cell culture incubator, and discard it after the virus is adsorbed for 1 hour. After washing the cell surface 3 times...
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