Traditional Chinese medicine composition for treating breast cancer, breast cyst, breast mass and breast pain
A technology for breast cysts and breast cancer, applied in the field of medicine, can solve the problems of loose cell connections, easy shedding, loss of breast cancer cells, etc., and achieve the effect of alleviating breast cysts, inhibiting the growth of cancer cells, and regulating the body's immunity
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Embodiment 1
[0017] Weigh 20 grams of oysters, 13 grams of turtle shells, 20 grams of shijianchuan, 8 grams of pangolins, 20 grams of piercing stones, 20 grams of seaweed, 20 grams of kelp, 12 grams of trigonum, 12 grams of curcuma, 20 grams of melon, and salvia miltiorrhiza 20 grams, 11 grams of earthworm, 8 grams of ground beetle, 20 grams of barbata, 20 grams of lobelia, 20 grams of black nightshade, 12 grams of tiankui seeds, 12 grams of zhebei, 12 grams of litchi core, 20 grams of codonopsis, astragalus 20 grams, 18 grams of Poria, 20 grams of Atractylodes macrocephala, 30 grams of Chinese yam, 30 grams of Prunella vulgaris, 12 grams of angelica, 13 grams of madder, 12 grams of Rhizoma Cyperus, 18 grams of Yuanhu, 10 grams of Shanzi mushroom, 10 grams of Chonglou, loofah 15 grams of network, 12 grams of Citrus aurantii, 20 grams of Toosendan, 10 grams of frankincense, 10 grams of myrrh, 30 grams of honeysuckle, 15 grams of jujube, 30 grams of coix seed, 10 grams of saponins, 13 grams o...
Embodiment 2
[0019] Weigh out 0.1 g of Add 50,000 ml of sterile water, sonicate for 30 minutes, and then filter through a 0.22 μm microporous membrane to obtain solution 2.
experiment example
[0021] MTT (purchased from Sigma Company) was dissolved in normal saline, prepared into a working solution of 5 mg / mL, and stored at -20°C; dimethylsulfoxide (DMSO) was produced by Xi'an Chemical Reagent Factory and was analytically pure.
[0022] Principle of MTT method
[0023] MTT is an acceptable H + yellow dye. In the mitochondrial respiratory chain of living cells, succinate dehydrogenase and cytochrome C can cleave the tetrazolium ring of MTT to produce blue-purple formazan crystals. Dead cells do not contain this dehydrogenase. The amount of Formazan crystals generated is directly proportional to the number of viable cells, and the DMSO solution of the crystals has a maximum absorption peak at 570nm. Therefore, the effect of the drug on cell proliferation can be evaluated by detecting the amount of formazan crystals.
[0024] testing method
[0025] Take the tumor cells in the logarithmic growth phase and adjust the cell density to 2×10 5 cells / mL, add 200 μL per ...
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