Euphorbia helioscopia extract, preparation method and application thereof
A technology of extract and lacquer, which is applied in the field of varnish extract and its preparation and application, and can solve the problems of multiple components, complex components, and complicated quality control
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Embodiment 1
[0039] The preferred of embodiment 1 extraction method
[0040] First of all, different extraction methods are used to extract the medicinal materials of Zeqi. Take 5 parts of medicinal materials of Zeqi, 25g each, and extract them with 95% ethanol for 2 to 3 times, 70% ethanol for 2 to 3 times, and 95% ethanol for impregnation. Extraction 2-3 times, ultrasonic extraction 2-3 times with 95% ethanol, decoction 2-3 times, combined extracts, filtered, evaporated to dryness under reduced pressure to obtain extracts E1, E2, E3, E4, E5 respectively.
[0041] The extracts E1, E2, E3, E4, and E5 obtained by the above-mentioned different extraction methods were screened for anti-psoriasis activity in vitro:
[0042] Materials and Reagents:
[0043] Zelacia extract; fetal bovine serum (Hangzhou Sijiqing Bioengineering Materials Co., Ltd.); DMEM high-glucose medium (Hyclone, USA); CCK-8 kit (Shanghai Beibo Biology).
[0044] In vitro model establishment:
[0045] TNF-α stimulates the ...
Embodiment 2
[0053] Example 2 Screening of anti-psoriasis effective parts of varnish
[0054] Take an appropriate amount of lacquer medicinal material 100g, reflux extraction with 90% ethanol for 2 to 3 times, combine the extracts, filter, and evaporate the solvent under reduced pressure to obtain the lacquer extract. Disperse the extract in a certain amount of water to form a suspension, sequentially extract the extract with petroleum ether, chloroform, ethyl acetate, and n-butanol at a volume ratio of 1:1, extract three times with each solvent, and combine the extracts , the solvent is evaporated to dryness under reduced pressure, that is, the petroleum ether part (F r a), the chloroform site (F r b), ethyl acetate site (F r c), n-butanol site (F r d).
[0055] The anti-psoriasis activity of the above parts was screened in vitro, and the results are shown in Table 2.
[0056] Table 2 Inhibitory effect of different parts of lacquer extract on keratinocyte proliferation
[0057]
...
Embodiment 3
[0059] Example 3 The preparation of the anti-psoriasis effective part of the lacquer extract
[0060] Take 15kg of sun-dried herbs, pour them into an extraction tank, add 60-80L of 90% ethanol aqueous solution, reflux extraction 4 times, 24 hours each time, combine the ethanol extracts obtained from 4 times, and filter. The ethanol extract is the total extract of lacquer after vacuum distillation and recovery of ethanol. After adding water to suspend, extract repeatedly three times with equal volumes of petroleum ether and chloroform successively, combine the extracts, and evaporate the solvent to dryness under reduced pressure to obtain the petroleum ether fraction Fra (150 g), which is a black semi-solid preparation. Corresponding auxiliary materials can be added to make cream. Chloroform part Frb (210g), this part is dark brown semi-solid preparation, can add corresponding adjuvant to make emulsifiable paste.
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