Recombinant production of steviol glycosides

Abstract

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

Description

[0001] This application is a divisional application of Chinese patent application CN201180038475.4, and the original application is an application that entered the Chinese national phase on February 4, 2013 with the international application number PCT / US2011 / 038967.

[0002] sequence listing

[0003] This application contains a Sequence Listing submitted via EFS-Web in ASCII format, which is hereby incorporated by reference in its entirety. The ASCII copy generated on June 2, 2011 is named 25933WO1.txt and is 483,406 bytes in size. technical field

[0004] The present disclosure relates to the recombinant production of steviol and steviol glycosides. In particular, the present disclosure relates to the production of steviol and steviol glycosides, such as rubusoside and / or rebaudioside A, by recombinant hosts such as recombinant microorganisms, plants or plant cells. The present disclosure also provides compositions containing the steviol glycosides. Background technique ...

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