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System and detection method for rapidly detecting catechol and derivatives thereof on site

A technology of catechol and its derivatives, which is applied in the field of colorimetric sensing array-smart phone-remote server system, can solve the problems of restricting practical application and unable to give detection results in time, and achieve simple and convenient experimental results, real-time Produce experimental results and ensure consistent results

Active Publication Date: 2016-06-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when used to detect actual samples on-site, the colorimetric sensing array method has to be sampled on-site, and then sent to the laboratory to collect photos with a camera or scanner and process data with a computer, so that it cannot be given in time. The detection results have limited its practical application

Method used

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  • System and detection method for rapidly detecting catechol and derivatives thereof on site
  • System and detection method for rapidly detecting catechol and derivatives thereof on site
  • System and detection method for rapidly detecting catechol and derivatives thereof on site

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Embodiment 1

[0056] A system for rapid on-site detection of catechol and its derivatives is based on a colorimetric sensing array-smart phone-remote server system, and the system includes a colorimetric sensing array, an image acquisition device, and a remote server;

[0057] The construction method of the system is carried out according to the following steps

[0058] (1) Preparation of combined probes for the detection of catechol and its derivatives: first prepare phosphate buffer, then

[0059] Finally, phenylboronic acid is dissolved in the above-mentioned phosphate buffer to prepare phenylboronic acid phosphate buffer; then the phenylboronic acid phosphate buffer is mixed with a pH indicator according to a certain ratio to form a combination for detecting catechol and its derivatives the solution of the probe;

[0060] Wherein the concentration of the phosphate buffer is 50mM, and the pH is 9; the phenylboronic acid phosphate buffer

[0061] The concentration of the liquid is 50mM;...

Embodiment 2

[0081] A method for rapidly detecting catechol and its derivatives on site:

[0082] This embodiment takes the detection of catechol, dopamine hydrochloride and 4-methylcatechol as examples, and uses a 2×2 colorimetric sensor array to predict the types and concentrations of the three targets. The present invention comprises the following steps:

[0083] (1) Phenylboronic acid and pH indicator as a combined probe: first prepare 50 mM phosphate buffer with a pH of 9, then dissolve phenylboronic acid in the above phosphate buffer to prepare 50 mM phenylboronic acid phosphate buffer. Mix 50mM phenylboronic acid phosphate buffer with four 0.1mg / mL pH indicators at a volume ratio of 1:1.5 to form four combined probes for detecting catechol and its derivatives.

[0084] The pH indicator is: xylenol orange, o-nitrophenol, rosebic acid, m-cresol violet.

[0085] (2) Preparation of colorimetric sensing array: Add the four probe solutions obtained by mixing phenylboronic acid and pH in...

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Abstract

The invention relates to a system and a detection method for rapidly detecting catechol and derivatives thereof on site and belongs to the technical field of chemical detection and analysis. The system is based on a colorimetric sensing array-a smartphone-a remote server. The detection method comprises the following steps: a combined probe for detecting catechol and derivatives thereof is prepared at first, and then is mixed with a target object to prepare the colorimetric sensing array; array images before and after a reaction are acquired by using the smartphone in an image acquisition device, and are subjected to digital processing to obtain different colorimetric sensing array chromatic aberration images after the reaction of catechol and the derivatives thereof and the combined probe; data of the colorimetric sensing array chromatic aberration images is uploaded to the remote server by using the smartphone, and a detection result is fed back into the phone through mathematical statistics and analysis, so that catechol and the derivatives thereof can be rapidly detected on site. The system and the detection method, provided by the invention, are low in cost and simple and convenient to operate, and an experiment result can be generated in real time; and the phone is convenient to carry and the sensing array can be used for on-site actual sample measurement.

Description

technical field [0001] The present invention relates to a system and a detection method for rapid on-site detection of catechol and its derivatives, in particular to a colorimetric sensing array-smart phone-remote server system for various catechols in environmental water samples The on-site rapid detection of derivatives thereof belongs to the technical field of chemical detection and analysis. Background technique [0002] Catechol and its derivatives belong to phenolic compounds, and their structures contain cis-dihydroxy groups. Because catechol and its derivatives are not only important pharmaceutical intermediates, but also important fine chemical intermediates, they are produced and used in large quantities in industry, such as catechol, 4-tert-butyl catechol, chlorine Acetyl catechol. Although catechol and its derivatives have great value, the phenolic pollution caused by product transportation leakage, sewage discharge and other reasons cannot be underestimated. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G06T7/00G06T7/40
CPCG01N21/78G06T7/0002G06T2207/10004G06T2207/10024
Inventor 李媛媛王赟韩娟鲍煦夏晋晨王蕾倪良张文莉
Owner JIANGSU UNIV
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