Method for rapidly identifying fruiting potential of edible fungus strains

A technology for edible mushrooms and strains, applied in the directions of botanical equipment and methods, applications, mushroom cultivation, etc., can solve the problems that mushroom bags cannot fruit normally, heavy workload, and complicated operation, so as to promote the production and development of edible mushrooms. , Improve work efficiency and simple operation

Inactive Publication Date: 2016-06-22
NINGDE NORMAL UNIV
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual production of edible fungi, it often happens that due to the nature of the bacteria, the fungus bag cannot produce mushrooms normally, causing huge economic losses to the growers. Small-scale cultivation and fruiting experiments to evaluate the fruiting potential of the strains, so as to avoid the huge risks that may be brought about in cultivation
[0005] The fruiting potential of t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1 Identification of fruiting potential of Lentinus edodes hybrid strain

[0019] 1) Weigh 200g of sawdust and 50g of bran in 1L of tap water, boil for 20min, filter, add water to the filtrate and dilute to 1L as a culture medium;

[0020] 2) Pack the culture medium into a 250mL conical flask in an amount of 150mL, and place a heat-resistant sponge with a size of 4cm×4cm×2cm in the bottle, seal it with 8 layers of gauze, and sterilize it in an autoclave at 121°C for 20min. ;

[0021] 3) After the medium is cooled, cut 2 pieces of 1cm×1cm from the slant of the activated Lentinus edodes hybrid strain, one of which is inoculated into the medium to make it grow in large quantities, and the other is inoculated in the center of the surface of the sponge position, as a support point for the formation of primordia or fruiting bodies;

[0022] 4) Incubate the inoculation bottle at 28°C and 160r / min for 5d on a shaker;

[0023] 5) After culturing in a shaker, place the...

Example Embodiment

[0025] Embodiment 2 The fruiting potential identification of a certain wild woody fungus isolated strain

[0026] 1) Weigh 200g of sawdust and 50g of bran in 1L of tap water, boil for 20min, filter, add water to the filtrate and dilute to 1L as a culture medium;

[0027] 2) Pack the culture medium into a 500mL wide-mouth bottle in an amount of 150mL, and place a heat-resistant sponge with a size of 4cm×4cm×2cm in the bottle, seal it with 8 layers of gauze, and sterilize it in an autoclave at 121°C. bacteria 20min;

[0028] 3) After the medium is cooled, cut 2 strain blocks with a size of 1cm × 1cm from the slant of the activated wild woody bacteria isolate, one of which is inoculated into the medium to make it grow in large quantities, and the other inoculated in The center position of the sponge surface, as the support point for the formation of primordium or fruiting body;

[0029] 4) Incubate the inoculated bottle at 28°C and 160r / min for 7d on a shaker;

[0030] 5) Af...

Example Embodiment

[0032] Example 3 Identification of fruiting potential of Pleurotus chinensis introduced species

[0033] 1) Weigh 200g of sawdust and 50g of bran in 1L of tap water, boil for 20min, filter, add water to the filtrate and dilute to 1L as a culture medium;

[0034] 2) Pack the culture medium into a 500mL wide-mouth bottle in an amount of 150mL, and place a heat-resistant sponge with a size of 4cm×4cm×2cm in the bottle, seal it with 8 layers of gauze, and sterilize it in an autoclave at 121°C. bacteria 20min;

[0035] 3) After the medium is cooled, cut two 1cm × 1cm strain blocks from the slanted surface of the activated Pleurotus chinensis, one of which is inoculated into the medium to make it grow in large quantities, and the other is inoculated on the surface of the sponge The central position of , as the support point for the formation of primordia or fruiting bodies;

[0036] 4) Incubate the inoculation bottle at 28°C and 160r / min for 5d on a shaker;

[0037] 5) After cu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for rapidly identifying the fruiting potential of edible fungus strains. An extracting solution obtained after sawdust and bran water extraction serves as a culture medium and then is charged into a wild-mouth bottle or a triangular flask, heat-resisting sponge with the specification of 4 cm * 4 cm * 2 cm is put into the bottles, two strain blocks with the specifications of 1 cm * 1 cm are respectively inoculated in the central positions of the culture medium and the sponge surface after high-temperature sterilization, shaking cultivation is performed for 5 days, then standing cultivation is performed for 10-12 days, and it is observed that primordial or young fruiting bodies can be formed at sponge inoculation positions. The edible fungus strains can be rapidly identified by adopting the method, especially the fruiting potential of lignicolous edible fungus strains, and the method can be applied to identification of the fruiting potential of wild edible fungi, hybrid strains and introduced strains before large-scale cultivation and fruiting tests and has a great significance on the promotion of production and development of edible fungi.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for quickly identifying the fruiting potential of edible fungus strains. Background technique [0002] Edible fungi are a class of large fungi that can produce edible fruiting bodies. They are rich in nutrients such as proteins, polypeptides, amino acids, carbohydrates, nucleotides, cellulose, trace elements, and vitamins. Food that people love. In addition to being a food with high nutritional value, edible fungi such as Ganoderma lucidum and shiitake mushrooms also have various pharmacological functions such as immune regulation, anti-oxidation, liver protection, anti-cancer, tumor cell inhibition, and anti-aging. Due to the high medical and food value of edible fungi and the continuous improvement of people's health awareness, the market demand for edible fungi continues to increase. Therefore, the production of edible fungi has great po...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 张维瑞刘盛荣苏贵平匡云波周修赵
Owner NINGDE NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap