Seedling culture medium of watermelon seedlings as well as preparation method and application of seedling culture medium

A technology for raising seedling substrates and watermelons, which is applied in planting substrates, botany equipment and methods, applications, etc., can solve the problems of high cost of raw materials and labor costs, high production costs, and expensive raw materials for nutrient solution preparation, and achieve low cost, The effect of increased root density and simple preparation method

Inactive Publication Date: 2016-06-22
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the price of raw materials is high, the production cost is high, and the preparation of nutrient solution requires a certain technical background, and the cost of raw materials and labor costs for the preparation of nutrient solution are relatively high.

Method used

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  • Seedling culture medium of watermelon seedlings as well as preparation method and application of seedling culture medium
  • Seedling culture medium of watermelon seedlings as well as preparation method and application of seedling culture medium
  • Seedling culture medium of watermelon seedlings as well as preparation method and application of seedling culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1:. Yeast fermentation process.

[0042] The yeast is first cultured in a liquid medium, and the components of the medium are: 20% potato, 2% glucose, and natural pH. When cultivating to the logarithmic growth phase, all of them are inoculated into the fermenter, and the fermentation medium composition is: 3% of malt powder, 4% of glucose, 2% of sucrose, and 0.3% of ammonium sulfate.

Embodiment 2

[0043] Embodiment 2: Actinomycetes fermentation process.

[0044] (1) Scrape a small amount of bacterial classification with inoculation stick, inoculate in liquid medium, liquid medium composition (g / L) is: soluble starch 20g, NaCl0.5g, KNO31g, K2HPO43H2O0.5g, MgSO47H2O0.5g, FeSO47H2O0. 01g, water 1000ml, pH7.4~7.6. Place it on a constant temperature shaker and incubate for 24 hours at 28°C and 240r / min.

[0045] (2) Inoculate the yeast in the logarithmic phase into the fermenter as a bacterial strain, and the fermentation medium is 3% of soluble starch, 0.8% of soybean peptone, 0.05% of dipotassium hydrogen phosphate, and 0.025% of magnesium sulfate.

Embodiment 3

[0046] Embodiment 3:: Lactobacillus acidophilus fermentation process.

[0047] (1) Scrape off a small amount of strains with an inoculation stick and inoculate them in a liquid medium. The composition of the liquid medium (g / L) is: glucose 20, peptone 10, yeast extract 5, beef extract 10, KH2PO42, NaAc5, MnS040. 2, MgSO40.2, ammonium citrate 2, Tween-801mL, pH 6.2-6.6. Place it on a constant temperature shaker and incubate for 24 hours at 28°C and 240r / min.

[0048] (2) Lactobacillus acidophilus in the logarithmic phase is inoculated into the fermenter as a strain, and the fermentation medium is 12% of whey powder, 3% of glucose, 0.5% of NH4H2P0, and the pH value is 6.2-6.6. Check the pH.

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PUM

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Abstract

The invention discloses a preparation method of a seedling culture medium of watermelon seedlings. The preparation method comprises the following steps: (1) uniformly mixing straws, rice husks, sawdust, biogas residues, mushroom residues and wine residues to obtain a fermentation medium; (2) adding a fermentation bacterial agent into the fermentation medium, and laminating, composting and fermenting to obtain a composted fermentation product, wherein the fermentation bacterial agent is mixed bacteria of saccharomycetes, actinomycetes, lactobacillus and bacillus subtilis; the saccharomycetes is pichia pastoris, the actinomycetes is streptomyces albus, the lactobacillus is lactobacillus acidophilus and the bacillus subtilis is bacillus vallismortis; (3) mixing the composted fermentation product with pearlite to obtain the seedling culture medium of the watermelon seedlings. The preparation method of the seedling culture medium of the watermelon seedlings has the advantages that the preparation method is simple, the cost is low, the cultured watermelon seedlings are stronger, the quality of the watermelon seedlings is good, the total dry weight of the watermelon seedlings is remarkably improved, the root density is remarkably improved and the like.

Description

[0001] technology field [0002] The invention belongs to the technical field of watermelon seedling cultivation, and in particular relates to a preparation method of a watermelon seedling cultivation substrate. Background technique [0003] Seedling cultivation is an important measure for the large-scale production of watermelon. The seedling substrate has developed from the initial soil seedling cultivation to inorganic substrate seedling cultivation and organic substrate seedling cultivation. Compared with soil substrate and inorganic substrate, organic substrate seedling cultivation has the advantages of high efficiency and strong seedlings. Inorganic substrate Nutrient solution needs to be watered during the seedling cultivation process. The preparation of nutrient solution requires the use of chemical raw materials and chemical reagents of chemical purity. In order to ensure the stability and effectiveness of trace elements such as iron and other metal elements, EDTA-Na ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05F15/00C05F17/00A01G31/00
CPCA01G24/00C05D9/00C05F5/00C05F9/04C05F17/00C05G3/00C05F5/002C05F5/008C05F11/00C05F11/08Y02E50/30Y02W30/40
Inventor 徐国华任丽轩王其传霍鸿巍祁红英董彩霞
Owner NANJING AGRICULTURAL UNIVERSITY
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