A strain of Penicillium sclerotinum poisonous to kill plant parasitic nematodes and its preparation method and application

Abstract

The invention discloses a Penicillium sclerotinum poisonous to kill plant parasitic nematodes, a preparation method and application thereof. The strain number of the Penicillium sclerotinum is D35, and its registration number in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms is CGMCC No. 12168. The active ingredient is a phytoparasitic nematode inhibitor of a substance dissolved in ethyl acetate extracted from a culture of Penicillium sclerotinum D35 with ethyl acetate, and the corrected mortality rate against Meloidogyne incognita is 100%. The average inhibition rate of Penicillium sclerotinum D35 on egg hatching of M. incognita was 99%. The solid fermentation culture of Penicillium sclerotiorum D35 has a control effect on cucumber root-knot nematode of 83.9% in 45 days without adding any additives, and still reaches 77.7% of cucumber root-knot nematode after 80 days. Cucumber seedlings treated with Penicillium sclerotiorum D35 had well-developed root system, few root knots, normal plant growth, and were safe to cucumber.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a Penicillium sclerotinum poisoning and killing plant parasitic nematodes and its preparation method and application. Background technique [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. Globally, the occurrence and damage of plant parasitic nematodes are becoming more and more serious with the development of agriculture and forestry and the change of farming system. It is estimated that the global annual loss of plant parasitic nematodes to agriculture and forestry production is as high as 157 billion US dollars (Abad P, et al. 2008. Genome sequence of the metazoan plant-parasitic nematode Meloidogyneincognita. Nature Biotechnology, 26: 909-915), The actual losses far exceed estimates. Furthermore, the direct or indirect impacts of nematodes on plants due to their interac...

AI Technical Summary

Problems solved by technology

At present, nematode control is still dominated by chemical control, but the surface of the nematode body is cuticle without cell structure, the nervous system is underdeveloped, the air permeability and water permeability of the nematode are poor, and the response to chemical substances is slow, so there are many chemical nematicides. It is a highly toxic pesticide, which is highly toxic to humans and animals and pollutes the environment. Many of them have been banned from being used in vegetables and fruits

Although there are some medium and low toxicity nematicides suitable for use in vegetable fields, there are few varieties, which adds difficulties to the control of nematodes. Therefore, it is imminent to develop efficient and safe bionematicides

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