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Non-enzyme glucose sensor and manufacturing method thereof

A glucose sensor and glassy carbon electrode technology, which is applied in the field of Pd-Ce-GC-based enzyme-free glucose sensor and its preparation, can solve the problems of insufficient sensitivity, insufficient composition and preparation process, and insufficient selectivity. High performance, high linear correlation, and good stability

Inactive Publication Date: 2016-06-29
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, due to the deficiencies in the composition and preparation process, the existing enzyme-free glucose sensor still has the defects of insufficient selectivity and insufficient sensitivity.

Method used

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  • Non-enzyme glucose sensor and manufacturing method thereof

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Experimental program
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Effect test

Embodiment 1

[0027] (1) Glassy carbon electrode pretreatment: Polish with 0.05μm alumina powder, rinse the polished electrode with deionized water to remove dirt, and then ultrasonically wash with 2mol / LNaOH, ethanol, 2mol / LHCl and deionized water .

[0028] (2) Deposit PS film: Drop-coat 5 μL of PS bead aqueous solution (uniform by ultrasound) on the pretreated glassy carbon electrode, place it in the dark at room temperature for 24 hours, and then put it in a vacuum oven at 70°C for one hour. The SEM image of the glassy carbon electrode covered by the PS bead film is as follows figure 2 shown.

[0029] (3) Preparation of Pd-Ce(PS)-GC electrode: preparation containing 0.8mMPdCl 2 , 0.2mMCeCl 3 、0.5MKNO 3 Electrodeposition bottom solution. The electrode deposited with PS film was placed in the solution prepared above, and the electrochemical reduction experiment was carried out at -0.3V, and the deposition electricity was 1×10 -2 c. Pd in ​​solution during this process 2+ 、Ce 3+ ...

Embodiment 2

[0033] (1) Glassy carbon electrode pretreatment: Polish with 0.05μm alumina powder, rinse the polished electrode with deionized water to remove dirt, and then ultrasonically wash with 2mol / LNaOH, ethanol, 2mol / LHCl and deionized water .

[0034] (2) Deposit PS film: Drop-coat 5 μL of PS bead aqueous solution (uniform by ultrasound) on the pretreated glassy carbon electrode, place it in the dark at room temperature for 24 hours, and then put it in a vacuum oven at 70°C for one hour.

[0035] (3) Preparation of Pd-Ce(PS)-GC electrode: preparation containing 0.8mMPdCl 2 , 0.2mMCeCl 3 、0.5MKNO 3 Electrodeposition bottom solution. The electrode deposited with PS film was placed in the above solution, and the electrochemical reduction experiment was carried out at -0.3V, and the deposition electricity was 1×10 -2 c. Pd in ​​solution during this process 2+ 、Ce 3+ be reduced to form Pd-Ce nanoparticles. Put the glassy carbon electrode with adsorbed Pd-Ce particles into tetrahy...

Embodiment 3

[0039] (1) Pretreatment of matrix glassy carbon electrode: Polish with 0.05μm alumina powder, rinse the polished electrode with deionized water to remove dirt, and then pass through 2mol / LNaOH, ethanol, 2mol / LHCl and deionized water in sequence Ultrasonic washing.

[0040] (2) Preparation of PS template: Drop-coat 5 μL of PS bead aqueous solution (uniform by ultrasound) on the pretreated glassy carbon electrode, place it in the dark at room temperature for 24 hours, and then put it in a vacuum oven at 70°C for one hour .

[0041] (3) Preparation of Pd-Ce(PS)-GC electrode: preparation containing 0.8mMPdCl 2 , 0.2mMCeCl 3 、0.5MKNO 3 Electrodeposition bottom solution. The electrode deposited with PS film was placed in the solution prepared above, and the electrochemical reduction experiment was carried out at -0.3V, and the deposition electricity was 1×10 -2 c. Pd in ​​solution during this process 2+ 、Ce 3+ be reduced to form Pd-Ce nanoparticles. Put the glassy carbon el...

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Abstract

The invention discloses an enzyme-free glucose sensor, a preparation method thereof, and a glucose concentration testing system composed of the sensor. The enzyme-free glucose sensor comprises a glassy carbon electrode as a substrate, a PS film deposited on the glassy carbon electrode and Pd-Ce particles adsorbed on the glassy carbon electrode. The preparation method of the enzyme-free glucose sensor comprises (10) glassy carbon electrode pretreatment; (20) deposition of PS film; (30) Pd-Ce(PS)-GC electrode preparation steps. Glucose concentration test system comprises working electrode, auxiliary electrode and counter electrode, and described counter electrode is a platinum sheet electrode, and auxiliary electrode is saturated calomel electrode, and described working electrode is based on Pd-Ce(PS)-GC enzyme-free glucose sensor . The non-enzyme glucose sensor and the glucose concentration test system composed of the present invention have good selectivity and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of glucose electrochemical detection equipment, in particular to an enzyme-free glucose sensor based on Pd-Ce(PS)-GC with good selectivity and high sensitivity and a preparation method thereof. Background technique [0002] Glucose detection is widely used in medicine, food, biotechnology, environmental science and other fields, and the research on its analysis and determination method is of great significance. Glucose determination methods mainly include chromatography, spectrometry and electrochemical methods. Among them, the electrochemical glucose sensor is widely used because of its simple device, rapid detection, high selectivity and sensitivity. In the electrochemical detection method, the performance of the glucose sensor is the key to the success of the detection. [0003] Common electrochemical glucose sensors are divided into two types: enzyme sensors and enzyme-free sensors. Enzyme-free sensor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/327G01N27/30
Inventor 杨晨王凯丽胡效亚
Owner YANGZHOU UNIV
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