Method for detecting intercepted amount of bactericide in cigarette end

Abstract

The invention discloses a method for detecting the interception amount of a fungicide in a cigarette butt. The cigarette butt includes a filter tip and residual shredded tobacco. One or more, including the following steps: select a sample cigarette, collect the filter after smoking the cigarette; disassemble the filter and shredded tobacco in the cigarette butt, and extract them separately; the first screw cap after vortex oscillation and freezing Add anhydrous magnesium sulfate, sodium chloride, sodium citrate and sodium dihydrogen citrate to the centrifuge tube and the second screw-cap centrifuge tube respectively, centrifuge after the third vortex oscillation, and freeze after centrifugation; ‑Propylethylenediamine purification: final analysis using LC‑MS / MS. The invention not only retains better extraction effect, but also does not dissolve the polypropylene fiber or acetate fiber in the filter tip, thereby reducing the difficulty of extracting the fungicide from the extraction solvent.

Description

technical field [0001] The invention belongs to the field of detection of pesticide residues, and relates to a detection method of bactericide interception in cigarette butts. Background technique [0002] Leaf spot and black stem are the most common bacterial diseases in tobacco, which have a great impact on the yield and treatment of tobacco. Propamocarb, carbendazim, rice blastazim, triadimenol and triadimefon are commonly used fungicides in the production of flue-cured tobacco, which can prevent and control tobacco leaf spot and black shank, and have the characteristics of high efficiency and low toxicity. Except for carbendazim, the other four indicators are included in the "China Tobacco Leaf Company's 2015 List of Pesticides Recommended for Tobacco Use" and are used in various tobacco areas. Due to their wide application, these fungicides are high residues in tobacco leaves. indicators, often detected in cured tobacco leaves. Residues of fungicides in tobacco leaves...

AI Technical Summary

Problems solved by technology

Previous researchers paid more attention to the smoke and filter transfer behavior of organophosphorus, pyrethroid and carbamate pesticides, and most of these pesticides are banned or are not recommended for use in tobacco, and the detection rate in tobacco is low

In addition, some researchers have paid attention to the smoke, ash and filter transfer behavior of maleic hydrazide, a budding agent used in tobacco. There are large differences in the transfer process of pesticides in the actual smoking process

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