Method for extracting perilla oil and ursolic acid from perilla seeds

A technology of ursolic acid and perilla seeds, which is applied in pharmaceutical formulations, fat oil/fat production, fat oil/fat refining, etc., can solve problems such as ursolic acid waste, reduce production costs, ensure pure naturalness, and reduce excessive effect used

Inactive Publication Date: 2016-07-06
王婧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In addition to a large amount of oil, perilla seeds also contain active substances such as ursolic acid. When extracting oil, ursolic acid and other substances have not been well utilized. The invention provides a method for refining perilla seed oil, and bearberry The acid extraction method completely solves the problem of a large amount of ursolic acid being wasted in the process of extracting the active ingredients of perilla

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] After crushing, 100 kg of perilla seeds and 300 kg of petroleum ether are extracted at room temperature to obtain petroleum ether extract and perilla residue, and the petroleum ether extract is concentrated under vacuum or reduced pressure at 60° C. to obtain perilla volatile oil. Add 400L of methanol to perilla residue Ⅰ, carry out reflux extraction at 60°C for 4 hours, filter 2 times with three layers of gauze, filter 1 time with ceramic membrane to get filtrate Ⅰ, take the filtrate Ⅰ and concentrate under vacuum or reduced pressure at 55°C to obtain 25L concentrated Liquid I. Adsorb the concentrated solution I on an LSA-10 macroporous resin column, wash the column with three times the column volume of 40% methanol aqueous solution, and then use three to five times the column volume of anhydrous methanol for column elution to obtain 80L wash Dehydration II. Concentrate the eluent II under vacuum or reduced pressure to 12L at 55°C, adjust its pH value to 8 with 0.1mol...

Embodiment 2

[0028] After crushing, 200 kg of perilla seeds and 500 kg of petroleum ether are extracted at room temperature to obtain petroleum ether extract and perilla residue. The petroleum ether extract is concentrated under vacuum or reduced pressure at 60° C. to obtain perilla volatile oil. Add 600L of methanol to perilla residue I and carry out reflux extraction at 65°C for 4 hours, filter twice with three layers of gauze and filter once with ceramic membrane to get filtrate I, take filtrate I and concentrate under vacuum or reduced pressure at 55°C to obtain 40L concentrated Liquid I. Adsorb the concentrated solution I on an LSA-10 macroporous resin column, wash the column with 50% methanol aqueous solution three times the column volume, and then elute the column with three to five times the column volume of anhydrous methanol to obtain 150L washing Dehydration II. Concentrate the eluate II under vacuum or reduced pressure to 20L at 55°C, adjust its pH value to 8 with 0.1mol / L sod...

Embodiment 3

[0030]After crushing, 400 kg of perilla seeds and 1000 kg of petroleum ether are extracted at room temperature to obtain petroleum ether extract and perilla residue. The petroleum ether extract is concentrated under vacuum or reduced pressure at 60° C. to obtain perilla volatile oil. Add 1500L of methanol to perilla residue I, carry out reflux extraction at 60°C for 4 hours, filter twice with three layers of gauze, and filter once with ceramic membrane to remove residue to obtain filtrate I, take filtrate I and concentrate under vacuum or reduced pressure at 55°C to obtain 100L concentrated Liquid I. Adsorb the concentrated solution I on the LSA-10 macroporous resin column, wash the column with 60% aqueous methanol solution three times the column volume, and then elute the column with anhydrous methanol three to five times the column volume to obtain 250L washing Dehydration II. Concentrate the eluent II under vacuum or reduced pressure to 40L at 55°C, adjust its pH value to ...

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PUM

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Abstract

The invention provides a method for extracting perilla oil and ursolic acid from perilla seeds, and obtaining ursolic acid and oleanolic acid crystals through methods such as petroleum ether leaching and column passing. The method can make full use of perilla, obtain perilla seed oil, ursolic acid and oleanolic acid products, effectively reduce the production cost, and make full use of two main active ingredients in perilla seed. Through the combination of resin and crystallization, the excessive use of chemical reagents is reduced, the pure nature of the product is guaranteed, and it can be safely provided to food, cosmetics and pharmaceutical industries.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and relates to a method for extracting perilla oil and ursolic acid from perilla seeds. Background technique [0002] Perilla, also known as Chisu, Fragrant Su, Baisu, etc., is an annual herb of the Lamiaceae Perilla genus, native to Myanmar, China and other eastern Asia. Perilla is a commonly used bulk medicinal material, which can be used all over the body. Perilla leaf has the functions of moving qi in the middle, expelling cold, and detoxifying fish and crabs. It is mainly used to treat colds and colds, cough and asthma, stagnation of spleen and stomach, vomiting, and poisoning of fish, shrimp and crab. Perilla stalk can widen the chest and sharpen the diaphragm, smooth the Qi and ease the fetus; perilla seed can treat cough, phlegm and dyspnea. Perilla is the first batch of medicinal and edible homologous plants included in the list. [0003] Perilla leaves contain 17 k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C11B1/10C11B1/04C11B3/00A61K36/535
CPCC07J63/008A61K36/535C11B1/04C11B1/10C11B3/00
Inventor 王婧
Owner 王婧
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