Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method and application of efficient cyhalothrin hapten

A high-efficiency cyhalothrin and hapten technology, applied in chemical instruments and methods, animal/human proteins, serum albumin, etc., can solve problems such as unfriendly and toxic environments, and achieve strong operability and simple process. , the effect of high yield

Inactive Publication Date: 2016-07-06
贵州勤邦食品安全科学技术有限公司
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Inspection and Quarantine Technology Center of Fujian Entry-Exit Inspection and Quarantine Bureau has obtained the authorization of the invention patent "Rapid Detection Method for Pyrethroid Pesticide Residues" (Patent No.: 200410155382.1). Pesticide residues are decomposed into cyanide-containing compounds. This invention uses strong acid and strong alkali reagents, and the products are cyanide-containing compounds, which are toxic and unfriendly to the environment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of efficient cyhalothrin hapten
  • Preparation method and application of efficient cyhalothrin hapten
  • Preparation method and application of efficient cyhalothrin hapten

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Synthesis and identification of lambda-cyhalothrin hapten

[0037] 1. Synthesis of lambda-cyhalothrin hapten

[0038] Weigh 0.42g (1mmol) of efficient cyhalothrin, 0.10g (1mmol) of succinic anhydride and 0.05g of aluminum trichloride respectively.

[0039] 1. Dissolve 0.42 g of lambda-cyhalothrin in dried methylene chloride, and pass through nitrogen for protection to obtain solution I;

[0040] 2. Add 0.10 g of succinic anhydride to solution I, stir and dissolve at room temperature;

[0041] 3. Add 0.01g aluminum trichloride solid to carry out catalytic reaction;

[0042] 4. TLC thin-layer plate monitors the progress of the reaction until there is no raw material or the raw material point is very shallow, and the reaction is stopped;

[0043] 5. Silica gel column purification, concentration to obtain the product, which is the hapten, such as figure 2 Shown, obtain product 0.205g, yield 48.8%

[0044] .

[0045] 2. Identification of lambda-cyhalothrin ...

Embodiment 2

[0073] Example 2: Synthesis and identification of lambda-cyhalothrin hapten

[0074] 1. Synthesis of lambda-cyhalothrin hapten

[0075] Weigh 1g (2.4mmol) of efficient cyhalothrin, 0.48g (4.8mmol) of succinic anhydride and 0.03g of aluminum trichloride respectively.

[0076] 1. Dissolve 1 g of lambda-cyhalothrin in dried methylene chloride, and pass through nitrogen for protection to obtain solution I;

[0077] 2. Add 0.48g of succinic anhydride to solution I, stir and dissolve at room temperature;

[0078] 3. Add 0.03g aluminum trichloride solid to carry out catalytic reaction;

[0079] 4. TLC thin-layer plate monitors the progress of the reaction until there is no raw material or the raw material point is very shallow, and the reaction is stopped;

[0080] 5. Silica gel column purification, concentration to obtain the product, which is the hapten, such as figure 2 Shown, obtain product 0.516g, yield 51.6%

[0081] .

[0082] 2. Identification of lambda-cyhalothrin h...

Embodiment 3

[0110] Example 3: Synthesis and identification of lambda-cyhalothrin hapten

[0111] 1. Synthesis of lambda-cyhalothrin hapten

[0112] Weigh 2.5g (6mmol) of efficient cyhalothrin, 1.8g (18mmol) of succinic anhydride and 0.12g of aluminum trichloride respectively.

[0113] 1. Dissolve 2.5 g of lambda-cyhalothrin in dried methylene chloride, and pass through nitrogen for protection to obtain solution I;

[0114] 2. Add 1.8g of succinic anhydride to solution I, stir and dissolve at room temperature;

[0115] 3. Add 0.12g aluminum trichloride solid to carry out catalytic reaction;

[0116] 4. TLC thin-layer plate monitors the progress of the reaction until there is no raw material or the raw material point is very shallow, and the reaction is stopped;

[0117] 5. Silica gel column purification, concentration to obtain the product, which is the hapten, such as figure 2 Shown, obtain product 1.26g, yield 50.4%

[0118] .

[0119] 2. Identification of lambda-cyhalothrin hap...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention provides a preparation method and application of efficient cyhalothrin hapten.A synthetic method of the efficient cyhalothrin hapten, provided herein, comprises: subjecting efficient cyhalothrin as a material and succinic anhydride to one-step reaction, and coupling hapten and carrier protein to obtain the efficient cyhalothrin hapten.Experimental results show that the synthetic method of the efficient cyhalothrin hapten is simple, the obtained efficient cyhalothrin hapten is higher in both yield and purity, an antigen hapten and carrier protein coupled herein may be used to immunize an experimental animal to obtain a monoclonal antibody good in specificity and sensitivity, an enzyme linked immunosorbent assay kit and test strip established using the antibody is convenient to use and economical, a detection method is efficient and accurate and available for detecting mass specimens, and the demand for field monitoring of efficient cyhalothrin hapten residue and screening of mass specimens can be met.

Description

technical field [0001] The invention relates to a preparation method and application of a residual hapten of lambda-cyhalothrin. Background technique [0002] Lambda-Cyhalothrin, English name Lambda-Cyhalothrin, also known as Aikonin, λ-cyhalothrin, belongs to pyrethroids, a hygienic insecticide with contact effect, chemical name: α-cyano-3 -Phenoxybenzyl-3-(2-chloro-3,3,3-trichlorofluoro-1-propenyl)-2,2-dimethylcyclopropanecarboxylate (Z)-(1R,3R) , S-ester and (Z), (1S,3S), 1:1 mixture of R-ester. It has contact killing effect and is a broad-spectrum insecticide that is extremely effective against environmental sanitation pests. It has the advantages of fast knockdown speed, strong knockdown force, and less dosage. It can eliminate vector pests that spread diseases and control various sanitary pests. Pyrethroids are known as a new breakthrough in insecticide pesticides and the third milestone in the history of insecticides. In agricultural production, it is widely used....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K14/765G01N33/68G01N33/531
CPCC07K14/435C07K14/765G01N33/531G01N33/68G01N2333/47G01N2333/765G01N2430/12
Inventor 冯才伟汪善良扶胜蔡韬陆苇谢体波刘红李平王大敏牛治存
Owner 贵州勤邦食品安全科学技术有限公司