A preparation method of hydrogel matrix for ultraviolet light 3D printing

A 3D printing, ultraviolet light technology, applied in the direction of prosthesis, tissue regeneration, medical science, etc., can solve the problems of secondary damage, absorption, and incomplete degradation of patients, so as to reduce the pain of patients, shape good, improve The effect of 3D printing efficiency

Inactive Publication Date: 2018-11-06
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method uses a mixed system of chitosan grafted with alkenyl groups and mercaptopolyvinyl alcohol. Since it cannot be completely degraded, it cannot be absorbed by human tissues. When it is transplanted into the body to achieve partial structure and function replacement, Surgical removal is also required, causing serious secondary damage to the patient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] A preparation method of a hydrogel matrix for ultraviolet light 3D printing, the preparation method is carried out in the following steps:

[0023] a. Preparation of Maleylated Hyaluronic Acid

[0024] Put hyaluronic acid and maleic anhydride in an aprotic solvent, the mass volume ratio of hyaluronic acid to aprotic solvent is 1:5-200, and the molar ratio of hydroxyl groups on the molecular chain of hyaluronic acid to anhydride groups of maleic anhydride is 1 : 0.1~20, stir evenly at room temperature, react under the condition of 25~80℃, the reaction time is 12~48 hours, after the reaction, add 1mol / L NaHCO 3 solution, adjust the pH of the mixed solution to 7-8, and dialyze the mixed solution for 2 days to form a maleylated hyaluronic acid solution. Under the condition of a pressure of 1-20 Pa, freeze-dry for 24-72 hours to obtain maleylated hyaluronic acid with a molar substitution degree of 0.05-0.3, and the aprotic solvent is dimethylformamide or dimethylacetamid...

Embodiment 1

[0038] Weigh 4g of hyaluronic acid and 0.41g of maleic anhydride, add them to 20mL of dimethylformamide, stir well at room temperature, and react at 25°C for 12 hours. After the reaction, add 1mol / L NaHCO 3 Solution, adjust the pH of the mixed solution to 7-8, dialyze the mixed solution, the dialysis time is 2 days, and freeze-dry the dialysate for 24 hours at a temperature of -50 ° C and a pressure of 1 Pa to obtain a molar substitution of 0.05 maleylated hyaluronic acid.

[0039] Weigh 2 g of maleylated hyaluronic acid, 2 g of bis-mercapto polyethylene glycol, and 0.05 g of 2-hydroxy-2-methyl-1-p-hydroxyethyl ether-based phenylacetone, and add them to 95.95 g of a pH-7.0 Na 2 HPO 4 -NaH 2 PO 4 In the buffer solution, mix uniformly at room temperature to obtain a UV 3D printing hydrogel matrix with a viscosity of 10,000 cps.

Embodiment 2

[0041] Weigh 4g of hyaluronic acid and 82.79g of maleic anhydride, add them into 800mL of dimethylacetamide, stir well at room temperature, and react at 80°C for 48 hours. After the reaction, add 1mol / L NaHCO 3 Solution, adjust the pH of the mixed solution to 7-8, dialyze the mixed solution, the dialysis time is 2 days, and freeze-dry the dialysate for 72 hours at a temperature of -50°C and a pressure of 20Pa to obtain a molar substitution of 0.3 maleylated hyaluronic acid.

[0042] Weigh 8 g of maleylated hyaluronic acid, 12 g of bis-mercapto polyethylene glycol, and 0.1 g of 1-hydroxycyclohexyl phenyl ketone, and add it to 79.9 g of K with a pH of 7.4. 2 HPO 4 -KH 2 PO 4 In the buffer solution, mix uniformly at room temperature to obtain a UV 3D printing hydrogel matrix with a viscosity of 100,000 cps.

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Abstract

The invention relates to a preparation method of hydrogel substrate, in particular to a preparation method of hydrogel substrate for ultraviolet 3D printing, and belongs to the technical field of preparation of bio-materials.The hydrogel substrate for ultraviolet 3D printing is prepared through grating vinyl radicals on hyaluronic-acid molecular chains, and mixing them with dithiol polyethylene glycol.The hydrogel substrate is quickly formed under irradiation of ultraviolet light by sufficiently utilizing the 'spotting' characteristic of thiol-alkene, inhibition of oxygen in the air or system can be thoroughly overcome, and 3D printing efficiency is greatly improved.Moreover, the hydrogel substrate is good in biological compatibility, and is completely degradable and absorbable by human tissues.The preparation method is simple, low in cost and easy to realize industrial production.

Description

technical field [0001] The invention relates to a method for preparing a hydrogel matrix, in particular to a method for preparing a hydrogel matrix for ultraviolet 3D printing, and belongs to the technical field of biomaterial preparation. Background technique [0002] Every year, a large number of patients need tissue or organ transplants for various reasons, but there is a serious shortage of donors. In order to solve this problem, researchers adopt tissue engineering technology, that is, tissue cells are used to form a three-dimensional space complex with biological scaffold materials in vitro, and after culture and expansion, a living tissue with vitality is formed, which is transplanted into the body to treat the lesion. Tissues undergo reconstruction of form, structure and function and achieve permanent replacement. Among them, the material selection and construction of biological scaffolds are particularly important. Requirements for the selection of bioscaffold mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G81/00C08B37/08C08J3/075A61L27/20A61L27/58A61L27/52
CPCA61L27/20A61L27/52A61L27/58A61L2430/40C08B37/0072C08G81/00C08J3/075C08J2387/00C08L5/08
Inventor 周应山董齐殷先泽杨红军柏自奎顾绍金徐卫林
Owner WUHAN TEXTILE UNIV
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