Green preparation method of scale insect slide specimen

A scale insect glass slide and a production method technology are applied in the field of production of scale insect glass slide specimens, and can solve the problems such as complicated production method of the scale insect glass slide, endanger human health, pollute the environment, etc. Long, easy-to-make effects

Inactive Publication Date: 2016-07-06
梁帆
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the deficiencies of the existing technology and solve the problems that the exis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Move the collected scale insects into 70% ethanol solution with a brush and soak for 2 hours to fix them, then place the fixed insect bodies under a stereo microscope, and use a scalpel to make an incision in the center of the back of the head and chest of the insect body. 1 / 2 of the length of the body, use a brush to move it into a 5mL beaker with 10% sodium hydroxide solution, place it on a heating plate, set the temperature of the heating plate to 110°C, and after heating for 10 minutes, move the body into a beaker filled with alkali. In a transparent colorimetric vessel with liquid, under a stereomicroscope, use tweezers and dissecting needle tools to clean the parasites until the contents are completely removed and the parasites become clean and transparent; use a small brush to move the parasites into a container filled with distilled water. Put it in a small beaker on a heating plate, set the temperature to 120°C, heat and soak for 10 minutes, then move the worms ...

Embodiment 2

[0022] The green manufacturing method of the scale insect slide specimen is made by the following steps:

[0023] 1). Immobilization of worms: move the live worms of scabies into 88% ethanol solution and soak for 2 hours to kill and fix the worms;

[0024] 2). Degreasing and transparent: place the fixed worm body under a stereo microscope, use a scalpel to make an incision in the center of the back of the cephalothorax of the worm body, the size of the incision accounts for 1 / 2 of the length of the worm body, and transfer it into Put the beaker in a 5mL beaker of sodium solution, then place the beaker on a heating plate, set the heating temperature to 100°C, and heat at a constant temperature for 10 minutes. When dyeing, the coloring of the worm body shall prevail. Move the worm body into a transparent cuvette filled with lye, and use any tool such as tweezers or dissecting needles to clean the worm body until the contents are completely removed and the worm body becomes clean...

Embodiment 3

[0032] The green manufacturing method of the scale insect slide specimen is made by the following steps:

[0033] 1). Immobilization of worms: move live scabies worms into 98% ethanol solution and soak for 2 hours to kill and fix worms;

[0034] 2). Degreasing and transparent: place the fixed worm body under a stereo microscope, use a scalpel to make an incision in the center of the back of the cephalothorax of the worm body, the size of the incision is about 1 / 2 of the length of the worm body, and add 9.8% hydrogen Put the beaker in a 5mL beaker of sodium oxide solution, then place the beaker in a water bath, set the heating temperature of the water bath to 110°C, and heat at a constant temperature for 10 minutes. The coloring of the worms will prevail during the later stage of dyeing. Move the worms into a transparent cuvette filled with lye, and use any tool such as tweezers or dissecting needles to clean the worms until the contents are completely removed and the worms bec...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses agreen preparation method of a scale insect slide specimen, belongs to the technical field of preparation methods of scale insect slide specimens and aims at solving the problem that an existing scale insect slide preparation method is complicated, pollutes the environment and harms human health.The green preparation method comprises the following steps of 1 insect body fixing, 2 degreasing until the insect body is transparent, 3 dealkalizing, 4 dyeing liquid preparation, 5 dehydration and color fixation and 6 slide sealing, wherein in the step 6, Canadian gum is dropwise added to a slide, an insect body is moved to the slide, attitude adjustment is performed, and then the slide is sealed, namely the scale insect slide specimen is obtained.The preparation method is simple and convenient, good in dyeing effect and long in service life and is suitable for preparation of the scale insect slide specimen.

Description

technical field [0001] The invention belongs to the technical field of preparation methods of insect slide specimens and relates to a preparation method of scale insect slide specimens. Background technique [0002] Scale insects, also known as scale insects, belong to the insect class of the phylum Arthropoda. Most scale insects are plant pests. Due to their strong reproductive capacity, they pose a serious threat to economic crops and garden plants, often causing plant leaf fall, fruit drop, branch death, and even the entire plant. Plants die; it is often fixed on the plant to cause damage, and the insect body is small, and it is often not noticed by people, and it is spread unintentionally by humans. At the same time, its reproduction rate is large, and once the scale insect is found, the damage is more serious. With the increasing frequency of international trade, the number of imported and exported seedlings and their fruits has increased, and the chances of spreading v...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/28
CPCG01N1/2813G01N2001/2833
Inventor 梁帆赵菊鹏林莉赵国栋周庆贤马骏
Owner 梁帆
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap