Method for analyzing polycyclic aromatic hydrocarbon by using micellar electrokinetic capillarychromatography

A technology of polycyclic aromatic hydrocarbons and capillary electrophoresis, which is applied in the field of analysis and detection, can solve the problems of not meeting the requirements of quality control and low detection sensitivity, and achieve the effects of good health, low analysis cost and rapid detection

Inactive Publication Date: 2016-07-06
JIANGNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the detection sensitivity of the traditional capillary electrophoresis method is low, which cannot meet the quality control requirements.

Method used

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  • Method for analyzing polycyclic aromatic hydrocarbon by using micellar electrokinetic capillarychromatography
  • Method for analyzing polycyclic aromatic hydrocarbon by using micellar electrokinetic capillarychromatography
  • Method for analyzing polycyclic aromatic hydrocarbon by using micellar electrokinetic capillarychromatography

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Experimental program
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Embodiment

[0022] 1. Materials and methods

[0023] (1) Test material:

[0024] Lotion in a certain brand of cosmetics.

[0025] (2) Instrument and chromatographic conditions:

[0026] TH-3100 high-efficiency capillary electrophoresis instrument.

[0027] Use uncoated quartz capillary (total length 65cm, effective length 50cm, column inner diameter 50μm) as the separation channel, enter the high conductivity zone (HCB) at 20s / 16Kpa, then inject the sample at 80s / 16Kpa, and detect with ultraviolet light The wavelength is 280nm, the operating voltage is -20kV, and the temperature is 20°C.

[0028] (3) Preparation of polycyclic aromatic hydrocarbon standard solution:

[0029] Accurately weigh six standard products, dissolve them in dichloromethane and ethanol to a 5mL volumetric flask, make a 2g / L stock solution, and store them in the dark at -18°C.

[0030] (4) Sample solution preparation:

[0031] Accurately weigh 2.00g of the cosmetic to be tested, add 10mL of n-hexane-acetone (1:1...

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Abstract

The invention provides a method for measuring a polycyclic aromatic hydrocarbon neutral molecule in cosmetics by using a Large Volume Sample Stacking-pH Dynamic link-micellar electrokinetic capillarychromatography (LVSS-DypH-MEEKC). The method employs the LVSS-DypH-MEEKC to analyze and detect a cosmetic extract, and obtains electrophoresis spectrograms of polycyclic aromatic hydrocarbon compounds of benzo[g, h, i]erylenesolution, dibenzo[a,h]anthracene, benzo[a]erylenesolution, benzo[b]fluoranthene, benzo[a]anthracene, and fluoranthene. The concentrations of the six substances are obtained according to a correction curvilinear equation. According to the invention, the concentrations of the benzo[g, h, i]erylenesolution, the dibenzo[a,h]anthracene, the benzo[a]erylenesolution, the benzo[b]fluoranthene, the benzo[a]anthracene, and the fluoranthene in the cosmetics can be analyzed and measured simultaneously. The method for analyzing polycyclic aromatic hydrocarbon by using micellar electrokinetic capillarychromatography can be used for detecting the polycyclic aromatic hydrocarbons and provides an effective reference for separation and enrichment of other neutral compounds with the same structure and property. The method is simple, rapid and high efficient. A buffer solution used in the method is mainly an aqueous solution without a large amount of organic solvents, has small pollution, and is environment friendly. The amount of requirement solvents and samples are small. The price of used hollow capillary columns is low, and the analysis cost is low.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and discloses a method for measuring polycyclic aromatic hydrocarbon neutral molecules in cosmetics by adopting large-volume sampling-pH dynamic connection-sweeping microemulsion capillary electrophoresis (LVSS-DypH-MEEKC). Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are mostly neutral molecules with high fat solubility and strong hydrophobicity, which can induce skin cancer, scrotal cancer and lung cancer, and are important environmental and food pollutants. "Hygienic Standards for Cosmetics" stipulates that benzo[g,h,i]pyrene, dibenzo[a,h]anthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[a]anthracene, Polycyclic aromatic hydrocarbons such as fluoranthene are banned substances in cosmetics. Currently, the main analytical techniques for PAHs are high performance liquid chromatography (HPLC) and gas chromatography (GC). Some PAHs cannot be directly ...

Claims

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Application Information

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IPC IPC(8): G01N27/447
Inventor 陈新曹玉华刘瑛
Owner JIANGNAN UNIV
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