Traditional Chinese medicine for treating swine eperythrozoonosis and preparing method and application thereof
A technology of porcine eperythrocytes and traditional Chinese medicine, which is applied in antibacterial drugs, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve problems such as death and economic losses, and achieve good curative effect, avoid residues, and simple preparation methods.
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Embodiment 1
[0017] Drying and pulverizing the rhizome of Lingshui Anthera to make a powder, so as to obtain the traditional Chinese medicine of the present invention.
[0018] When in use, the traditional Chinese medicine is added to the pig feed, and fed twice in the morning and evening, each 50g crude drug, for 3 consecutive days.
[0019] Or when in use, use warm water (35°C) to make the powder into a paste, which is in a suspension state, and it can be fed twice in the morning and evening, 50g of crude drug each time, for 3 consecutive days.
Embodiment 2
[0021] in vitro susceptibility test
[0022] Add 1 drop of pig blood suspension infected with eperythrozoonosis on 4 slides, then add 0.05g / L Xuechongjing, 0.05g / L oxytetracycline hydrochloride, 1.0g crude drug / L One drop each of Lingshui Anluo decoction and normal saline, mix well and immediately observe under a high-power microscope.
[0023] judgement standard:
[0024] Eperythrozoon detached from the surface of red blood cells and Eperythrozoon in the plasma stopped shaking, it was judged as drug sensitive.
[0025] Drug sensitivity test results:
[0026] Xuechongjing and oxytetracycline hydrochloride make the Eperythrozoon fall off from the red blood cell after 2 minutes, and the Eperythrozoon in the plasma stops shaking after 3 minutes; Lingshui Anluo water decoction makes the Eperythrozoon fall off from the red blood cell after 4 minutes Afterwards, the eperythrozoon in the plasma stops shaking. After 20 minutes of normal saline, it still adheres to the surface of r...
Embodiment 3
[0028] Chinese medicine of the present invention treats the clinical trial of porcine eperythrozoonosis:
[0029] 1. Materials and methods:
[0030] 1.1 Preparation of test drugs:
[0031] Medicine: the traditional Chinese medicine prepared in Example 1 of the present invention.
[0032] 1.2 Test animals:
[0033] Selected 127 diseased pigs that were naturally infected with Eperythrozoon. The diseased pigs mainly showed loss of appetite and fatigue, body temperature rose to 40-41 degrees Celsius, conjunctiva first flushed, then pale and yellow, and ears, limbs, and abdomen Wait for the skin to have reddish-purple spots. Eperythrocytic bodies can be seen on blood microscopy.
[0034] 1.3 Usage and dosage:
[0035] For sick pigs with normal diet, add it to the feed sooner or later, 50g of crude drug each time. For sick pigs with poor appetite, make Lingshui Anluogen powder into a paste with warm water and give it to the pigs, 50g crude drug each time. Use it for 3 consecu...
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