Pharmaceutic preparation for treating lung cancer and application of pharmaceutic preparation
A pharmaceutical preparation, a technology for lung cancer, applied in the field of medicine, can solve the problems of unsatisfactory use and curative effect, unable to grasp the root cause, difficult to cure, etc., and achieve the effects of good therapeutic effect, low cost, and no toxic and side effects
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Embodiment 1
[0084] Embodiment 1: animal model test
[0085] In the existing therapeutic field, the inventor retrieved and found that the combined use of fluorouracil + vincristine + cyclophosphamide in mice is a better western medicine treatment method. This animal experiment uses it as a drug for the treatment of lung cancer in mice. Invented positive control test group.
[0086] Urethane is an anesthetic that can be used to induce the occurrence of lung cancer. It is often used as an inducer in animal models of lung cancer. Therefore, the present invention uses urethane as an inducer.
[0087] 1.1. Animal model establishment and administration
[0088] Female Kunming mice, 18-22g, 50, received intraperitoneal injection of urethane 800mg / kg twice a week for 5 consecutive weeks. Mice are divided into 5 groups at random, every group of 10: model group, positive control test I group (0.1mg+0.05mg+0.1mg), Chinese medicine composition high dose group (8g / kg), Chinese medicine composition mi...
Embodiment 2
[0110] Embodiment 2: clinical trial
[0111] 2.1. General Information
[0112] From 2013 to 2015, our hospital received a total of 88 lung cancer patients, who were divided into two groups, namely the experimental group and the observation group. The conditions of the two groups were basically the same, and there were significant differences in age and gender (P>0.05).
[0113] 2.2. Judgment criteria
[0114] Patients may be asymptomatic in the early stage, and most of them are found during chest X-ray examination. After tumor enlargement, cough, sputum, bloody sputum or a small amount of hemoptysis often occur, and secondary infection may have purulent sputum, fever, Loss of appetite, emaciation, etc., when there is a chest X-ray examination, it is found that there is a mass in the lungs, and often cough, expectoration, blood in the sputum or a small amount of hemoptysis, secondary infection may have purulent sputum, and fever, Those with symptoms of loss of appetite and we...
Embodiment 3
[0132] Example 3: Toxicity Verification
[0133] 3.1. Acute toxicity test
[0134] Tested Chinese medicine preparation: the capsules produced in Examples 4-7 of the present invention were added with purified water at a ratio of 1:2 to make a solution for later use. Experimental animals: normal grade Kunming mice, weighing 20g±5g, male and female, all female mice are sterile.
[0135] Mice gavaged the solution prepared by Chinese medicine capsules of the present invention, when the gavage dose reached 735.5g crude drug / kg dose, the mice showed a slight activity reduction after administration, and returned to normal in about 1 hour, and observed continuously for 7 days after administration. None of the animals died, and their general condition, diet, water intake, urination and weight gain were normal.
[0136] The test results showed that the maximum dosage of the capsules produced in Examples 4-7 was 735.5 g crude drug / kg / d (LD50>735.5 g crude drug / kg). The total amount of ...
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