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Culture medium and cultivation method for producing lincomycin by fermenting streptomyces lincolnensis

A fermentation medium, the technology of Streptomyces lincoeum, applied in the field of fermentation, can solve problems such as easy carbonization, darkening of the medium solution color, unfavorable decolorization treatment, etc.

Active Publication Date: 2016-07-13
HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1 Glucose is added to the medium, part of the glucose is easily carbonized under high temperature sterilization conditions, the color of the medium solution becomes darker, and the quality of the culture is reduced
[0005] 2 Relevant domestic literature reports some contents of lincomycin seed medium, fermentation medium formula and cultivation process, but cannot achieve industrialized mass production
[0006] 3 Amino acids are added to some domestic lincomycin fermentation production processes, which affects the quality of the fermentation broth and is not conducive to decolorization
[0007] 4 Domestic lincomycin production titer is generally maintained at about 9000u / ml, which is relatively low
[0008] 5 The cycle of lincomycin fermentation and cultivation is longer, generally around 160h

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Primary seed culture: medium volume is 1m 3 .

[0066] Primary seed medium group:

[0067] Wheat bran 14kg, maltose 6kg, earthworm powder 3kg, silk fibroin powder 4kg, corn steep liquor 7L, biotin 0.08kg, urea 0.04kg, light calcium carbonate 1kg, maltase 0.001kg.

[0068] First, sterilize the primary seed culture medium, cool it to 25°C, and keep the pressure with sterile air, and then transfer 1.6L of the cultured Streptomyces lincoeum mother bottle fermentation broth into the primary seed tank for cultivation under flame protection. Tank pressure 0.03-0.05MPa; tank temperature 29-30°C; air flow: 0-4h, using air stirring instead of mechanical stirring; 5h-transplant: 50m 3 / h; stirring speed 50r / min. After the primary seed cultivation is completed, transplant the seeds when the cell concentration is 20%, the pH value is 7.5, and the cultivation time is 30 hours.

[0069] Secondary seed culture: medium volume is 10m 3 .

[0070] Wheat bran 200kg, maltose 90kg, ear...

Embodiment 2

[0089] Primary seed culture: medium volume is 1m 3 .

[0090] Primary seed medium group:

[0091] Wheat bran 15kg, maltose 7kg, earthworm powder 4kg, silk fibroin powder 5kg, corn steep liquor 8L, biotin 0.09kg, urea 0.05kg, light calcium carbonate 2kg, maltase 0.002kg.

[0092] First, sterilize the primary seed culture medium, cool it to 27°C, and keep the pressure with sterile air, and then transfer 1.7 L of the cultured Streptomyces lincoeum fermented liquid into the primary seed tank under flame protection. Tank pressure 0.03-0.05MPa; tank temperature 29-30°C; air flow: 0-4h, using air stirring instead of mechanical stirring; 5h-transplant: 52m 3 / h; stirring speed 53r / min. After the primary seed culture is completed, the bacteria concentration is 22%, the pH value is 7.8, and the culture time is 32 hours.

[0093] Secondary seed culture: medium volume is 10m 3 .

[0094] Wheat bran 210kg, maltose 100kg, earthworm powder 70kg, silk fibroin powder 90kg, casein 80kg, c...

Embodiment 3

[0113] Primary seed culture: medium volume is 1m 3 .

[0114] Primary seed medium group:

[0115] Wheat bran 16kg, maltose 8kg, earthworm powder 5kg, silk fibroin powder 6kg, corn steep liquor 9L, biotin 0.1kg, urea 0.06kg, light calcium carbonate 3kg, maltase 0.003kg.

[0116]First, sterilize the primary seed culture medium, cool it to 28°C, and keep the pressure with sterile air, and then transfer 1.8 L of the cultured Streptomyces lincoeum mother bottle fermentation broth into the primary seed tank for cultivation under flame protection. Tank pressure 0.03-0.05MPa; tank temperature 29-30°C; air flow: 0-4h, using air stirring instead of mechanical stirring; 5h-transplant: 55m 3 / h; stirring speed 55r / min. After the primary seed culture is completed, the bacteria concentration is 23%, the pH value is 8.0, and the culture time is 33 hours.

[0117] Secondary seed culture: medium volume is 10m 3 .

[0118] Wheat bran 220kg, maltose 110kg, earthworm powder 80kg, silk fibro...

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PUM

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Abstract

The invention relates to a culture medium and a cultivation method for producing lincomycin by fermenting streptomyces lincolnensis. By the use of a seed culture medium formula, a fermentation culture medium formula and a control process which are provided by the invention, the fermentation unit for large-scale production of lincomycin can be increased, and the fermentation cost can be reduced; furthermore, the environmental influence on raw and auxiliary materials can be reduced to the maximum extent; sufficient supply of the materials is guaranteed, and efficient production of lincomycin is realized.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a culture method for producing lincomycin by fermentation of Streptomyces lincoeum. Background technique [0002] Lincomycin, also known as lincomycin and lincomycin, is a broad-spectrum antibiotic produced by Streptomyces lincoeum through aerobic fermentation and belongs to the lincomycin class. It acts on the 50S subunit of the ribosome of sensitive bacteria, prevents the extension of the peptide chain, thereby inhibiting the protein synthesis of bacterial cells, and has strong antibacterial activity against most Gram-positive bacteria, especially sensitive to Staphylococcus aureus and Streptococcus pneumoniae. It has good tissue permeability, slow development of drug resistance, good curative effect on infection of respiratory system, bone and cartilage tissue, and low toxicity, so it is an important clinical antibiotic. [0003] The dome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/64C12R1/565
Inventor 任勇徐淑芬
Owner HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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