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Microorganism self-assembly nanometer material and preparation method and application thereof

A technology of nanomaterials and microorganisms, applied in the field of microbial self-assembled nanomaterials and its preparation, to achieve the effects of uniform particle size distribution, high adaptability, and simple operation

Active Publication Date: 2016-07-20
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reports about the self-assembly of nano-calcium-phosphorus materials by microorganisms so far, so the self-assembly of nano-calcium-phosphorus materials through microbial technology is still a technical problem that needs to be solved in this field.

Method used

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  • Microorganism self-assembly nanometer material and preparation method and application thereof
  • Microorganism self-assembly nanometer material and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0034] Example 1 Isolation and identification of Shewanella sp. CF8-6

[0035] The screening and isolation process of Shewanella sp. CF8-6 is as follows:

[0036] (1) Several bacterial strains were isolated from the South China Sea mud, inoculated part of the strains isolated from the South China Sea mud into seawater LB liquid medium, and cultivated at 25°C and 200rpm for 24 hours to obtain an activated bacterial liquid;

[0037] (2) Inoculate the activated bacterial liquid obtained in the above step (1) into simulated high-salt wastewater at a ratio of 10%, cultivate it at 25°C and 200rpm, and select the one whose removal rate is higher than 90% in 12h and 24h. Several strains were re-screened;

[0038] (3) Screen the strains obtained in step (2) again according to steps (1) and (2), increase the sampling time points, select a strain with the best phosphorus removal speed and phosphorus removal rate, and name it CF8-6.

[0039] Components of the above-mentioned seawater LB...

Embodiment 2

[0046] The preparation of embodiment 2 microbial self-assembly nanomaterials

[0047] Utilize Shewanella strain (Shewanella sp.) CF8-6 to prepare the method for nanomaterial as follows:

[0048] (1) Cultivate the strain Shewanellasp.CF8-6 in seawater LB liquid medium at 25°C and 200rpm for 24 hours to obtain an activated bacterial liquid; centrifugation parameters of the activated bacterial liquid: centrifuge at 10000rpm for 10min;

[0049] (2) Centrifuge and wash the bacterial solution obtained in step (1) and put it into simulated waste water at a ratio of 10% (v / v), cultivate it for 48 hours at 25°C and 200rpm, and then centrifuge to obtain bacterial cells, nano Particles are distributed on and around the cell surface. The centrifugal speed is 4000rpm, and the time is 10 minutes;

[0050] (3) Wash the bacterium containing nanoparticles obtained in step (2) twice with deionized water at a rotation speed of 4000 rpm for 15 minutes. Then take it for transmission electron mi...

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Abstract

The invention discloses a microorganism self-assembly nanometer material and a preparation method and application thereof.In particular, according to the microorganism self-assembly nanometer material and the preparation method and application thereof, a Shewanella sp.strain CF8-6 which can efficiently remove phosphorus in high-salinity wastewater under an aerobiotic condition and meanwhile can synthesize calcium-phosphorus nanometer particles through raw materials in the wastewater under a low-saturability condition is utilized, phosphorus in the wastewater is removed, a calcium-phosphorus nanometer material is synthesized, and particularly the calcium-phosphorus nanometer particles are synthesized under the low phosphorus saturability condition.The method and the nanometer material prepared through the method play an important role in the fields of environmental pollution removal, biomedicine and the like.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation, and in particular relates to a microbial self-assembled nanomaterial and its preparation method and application. Background technique [0002] Nanomaterials refer to materials in which at least one of the three-dimensional materials is in the nanoscale range or composed of them as basic units. Nanomaterials have some unique characteristics such as surface effects, small size effects, and quantum effects, so they are widely used in energy, catalysis, biosensors, biomedicine and other industries. The traditional physical and chemical synthesis methods of nanomaterials mainly include two-phase method, reverse microemulsion method, photochemical synthesis method, electrode electrolysis method, heating method, ultrasonic method, etc., but these traditional synthesis methods have various insurmountable defects. For example, the price of raw materials is high, the energy consumption is high, the...

Claims

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Application Information

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IPC IPC(8): C12N1/20B82Y30/00C02F3/34A61K47/02A61L27/12A61L27/50C12R1/01C02F101/20
CPCA61K47/02A61L27/12A61L27/50A61L2430/02B82Y30/00C02F2101/20C12N1/20C12N1/205C12R2001/01
Inventor 周维芝王艳茹蒋丽
Owner SHANDONG UNIV
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