Reagent kit for quality evaluation of sperm after in vitro capacitation and use method of reagent kit

Abstract

The invention provides a reagent kit for quality evaluation of sperm after in vitro capacitation and a use method of the reagent kit. The reagent kit is stuffed with a detection buffer solution 1, a BWW culture solution, an in vitro capacitation solution, a fluorescent reagent, a sialidase standard stock solution and a detection buffer solution 2, wherein the detection buffer solution 1 is a phosphate buffer solution or the BWW culture solution; the detection buffer solution 2 is the phosphate buffer solution containing 0.05mmol / l sodium acetate and 0.25<mu>g / <mu>l bovine serum albumin. Through in vitro capacitation of the sperm, the activity of sialidase in a capacitation solution can be detected, so that a diagnosing basis and clinical consultation can be provided for patients suffering from male sterility of unknown clinical aetiology.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a kit for assessing sperm quality after in vitro capacitation and a use method thereof. Background technique [0002] With the continuous development of social industry, the total incidence of infertile couples increased significantly. In 2004, the statistics of the European Society of Reproduction: 25% of couples of childbearing age were unable to conceive within one year, 15% of them sought medical treatment, and 50% were infertility caused by male factors. The causes of male infertility are sexual dysfunction (1.7%), varicocele (12.3%), reproductive tract infection (6.6%), congenital dysplasia (2.1%) acquired disease (2.6%), endocrine disorders (0.6%), immune factors (3.1%), and other abnormalities (3%), but up to 60% to 75% of patients cannot find the cause, which is called idiopathic male infertility, and they only show oligospermia, Abnormal sperm qu...

AI Technical Summary

Problems solved by technology

However, the method includes the expression detection of sperm sialidase NEU1 / 3, which requires a fluorescence microscope or flow cytometer, and has the following disadvantages: 1) The technical process is complicated; 2) It requires high skills and experience in experimental equipment and operators; 3 ) Not suitable for use in clinical laboratories and routine technicians; 4) Use abcam's kit, which is expensive

In addition, because the number of sperm in different samples is different, the value of expressed protein is different, and the corresponding enzyme activity value is also different. Therefore, it is not scientific to simply compare the enzyme activity values ​​​​of different samples.

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