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Rapid saccharin sodium detection kit and detection method thereof

A detection kit and detection method technology are applied in the preparation of test samples, material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., which can solve the problem of expensive equipment and inconsistent detection gradients. It can reduce the probability of false positives, be easy to store and transport, and improve the detection sensitivity.

Active Publication Date: 2016-07-20
GUANGZHOU ANNUO FOOD SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-performance liquid chromatography has the advantages of high separation efficiency and high sensitivity. Using the standard control method, the content of sodium saccharin in the sample can be detected very accurately, but the equipment is expensive and bulky, and it is difficult to achieve rapid on-site detection
Except for methylene blue extraction photometry, other methods cannot meet the requirements of on-site rapid detection due to complicated sample extraction and separation, or because they are easily affected by factors such as food ingredients
[0006] The rapid detection kits for sodium saccharin of other companies on the market are based on the detection principle of methylene blue extraction photometry, but the extraction agent used is pure chloroform. This method has poor sensitivity, and the detection gradient is not obvious. The color difference can only be observed when the value is very large, and it is difficult for market users to accurately judge whether the detected value is within the qualified range, which brings confusion to market users

Method used

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  • Rapid saccharin sodium detection kit and detection method thereof
  • Rapid saccharin sodium detection kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Sprite, specification 500ml;

[0031] The present embodiment carries out rapid detection to the sodium saccharin content in Sprite, and its specific steps are as follows:

[0032] Take 1ml of chloroform and ethanol test solution into the reaction bottle, add 2ml of Sprite that has been shaken to remove carbon dioxide into the reaction bottle with the extraction agent, add dropwise 0.1ml of methylene blue solution to a concentration of 4*10 -3 mol / l, hydrogen ion concentration of 0.01mol / l methylene blue solution and sulfuric acid mixed solution, tighten the cap of the reaction bottle, shake vigorously for about 1 minute to mix the two phases, let stand until clear stratification and observe the lower layer Color development of the solution. If it is blue, it can be judged that there is sodium saccharin in the Sprite of this embodiment. The darker the color, the higher the sodium saccharin content. Compare the color with the standard color card to obtain t...

Embodiment 2

[0033] Embodiment 2: Coca-Cola, specification 500ml;

[0034] In this embodiment, the sodium saccharin content in Coca-Cola is rapidly detected, and its specific steps are as follows:

[0035] Take 3ml of chloroform and ethanol test solution into the reaction bottle, add 3ml of Coca-Cola that has been shaken to remove carbon dioxide into the reaction bottle with the extraction agent, add dropwise 0.5ml of methylene blue solution with a concentration of 0.05mol / l, The sulfuric acid mixed solution of methylene blue solution with a hydrogen ion concentration of 0.05mol / l, tighten the cap of the reaction bottle, shake vigorously for about 1 minute to mix the two phases, let stand until the layers are clearly separated, and then observe the color development of the lower solution . If it is blue, it can be determined that there is sodium saccharin in the Coca-Cola of this embodiment. The darker the color, the higher the sodium saccharin content. Compare the color with the standard...

Embodiment 3

[0036] Embodiment 3: Fanta, specification 500ml;

[0037] In this embodiment, the sodium saccharin content in Fanta is rapidly detected, and the specific steps are as follows:

[0038]Take 10ml of chloroform and ethanol test solution into the reaction bottle, add 10ml of Fanta that has been shaken to remove carbon dioxide, and add 2ml of methylene blue solution with a concentration of 0.16mol / l, hydrogen ion The sulfuric acid mixed solution of methylene blue solution with a concentration of 4mol / l, tighten the cap of the reaction bottle, shake it vigorously for about 1 minute to mix the two phases, let it stand until it is clearly separated, and then observe the color development of the lower layer solution, it is blue It can be judged that there is sodium saccharin in the fanta of this embodiment. The darker the color, the higher the sodium saccharin content. Compare the color with the standard color card to obtain the sodium saccharin concentration.

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Abstract

The invention provides a rapid saccharin sodium detection kit which is characterized by comprising an extracting agent, an acid solution of methylene blue and accessories such as a reaction bottle, a suction tube and a sampling spoon, wherein the extracting agent is a mixed reagent of trichloromethane and ethanol; the volume percentage of the ethanol in the extracting agent is 1-2%; the concentration of the methylene blue solution in the acid solution of the methylene blue is 4*10<-3>-0.16mol / l; the acid medium in the acid solution of the methylene blue is one of acid solutions such as sulfuric acid and hydrochloric acid; the hydrogen ion concentration of the acid medium is 0.01-4mol / l. A detection method of the rapid saccharin sodium detection kit adopts the mixed reagent of trichloromethane and ethanol as the extracting agent, so that the detection sensitivity is greatly improved, detection values have large developing gradients, whether a detected sample is within a qualification or not can be very accurately judged, and moreover as the stability of the trichloromethane is improved and the volatility is degraded, the rapid saccharin sodium detection kit is relatively easy to preserve and transport.

Description

technical field [0001] The invention relates to a rapid detection kit for sodium saccharin and a detection method thereof. Background technique [0002] The chemical name of saccharin is o-benzoylsulfonimide, and the commercial saccharin sold in the market is actually a soluble sodium salt of o-benzoylsulfonimide, referred to as sodium saccharin. Sodium saccharin is a synthetic sweetener commonly used in the food industry, and has the longest history of use, but it is also the most controversial synthetic sweetener. The sweetness is 300-500 times sweeter than sucrose, it is not decomposed in the living body, and is excreted by the kidneys. However, its toxicity is not strong, and the controversy is mainly due to its carcinogenicity. [0003] The raw materials for making saccharin mainly include toluene, chlorosulfonic acid, o-toluidine, etc., all of which are petrochemical products. Toluene is volatile and combustible, and may even cause an explosion. Ingestion of a large...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N1/34
CPCG01N1/34G01N21/78
Inventor 杨鹏博马丽张陇清杨松林王军
Owner GUANGZHOU ANNUO FOOD SCI & TECH CO LTD