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Fast detecting method of vitamin K1 used for preventing coupling hemorrhage after vaccine injection

A detection method and vitamin technology, applied in the field of electrochemical analysis, can solve the problems of poor enrichment and reproducibility of solid electrodes, poisonous mercury vapor, etc., and achieve the effect of increasing specific surface area and enhancing adsorption characteristics

Inactive Publication Date: 2016-07-20
ZHONGYI ANKE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the traditional electrochemical analysis method are: first, mercury electrodes are often used, although the reproducibility is good, but mercury vapor is poisonous; second, the enrichment amount and reproducibility of solid electrodes are poor

Method used

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  • Fast detecting method of vitamin K1 used for preventing coupling hemorrhage after vaccine injection
  • Fast detecting method of vitamin K1 used for preventing coupling hemorrhage after vaccine injection
  • Fast detecting method of vitamin K1 used for preventing coupling hemorrhage after vaccine injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The glassy carbon electrode is polished with 0.1~0.2μm aluminum oxide powder, the nano-titanium dioxide is prepared according to the concentration of 12g / L, the particle size of the nano-titanium dioxide is between 15 and 17nm, and the ultrasonic method is used to disperse the titanium dioxide solution for 30 minutes. A milky white dispersion is formed. In the experiment, the thickness of the nano-titanium dioxide film has an impact on the peak current, such as figure 1 It can be seen that when the titania dispersion is gradually increased from 5 μl to 15 μl, the dissolution peak current gradually increases, but after 15 μl, the current decreases instead, so 15 μl dispersion is selected as the optimal coverage amount. The specific method is to drop 15 μl of the above-mentioned dispersion liquid on the surface of the glassy carbon electrode, air-dry at room temperature, and repeat twice. The prepared titanium dioxide film modified glassy carbon electrode was used to det...

Embodiment 2

[0024] Establish a standard curve for the determination of vitamin K content and investigate the range of determination.

[0025] Take an appropriate amount of vitamin K1 standard stock solution, and use 5.0mol / L NaAc-HAc solution to prepare vitamin K1 content of 0.2ng / ml, 0.4ng / ml, 0.6ng / ml, 0.8ng / ml, 1.0ng / ml, 1.2ng / ml, 1.4ng / ml, 1.6ng / ml of a series of different vitamin K1 content standard solutions.

[0026] Take 5.0mol / L NaAc-HAc10~15ml of supporting electrolyte and add it into the measuring cup, blow high-purity nitrogen gas for 10min to remove oxygen, scan the blank supporting electrolyte in the potential range of -0.3V~-0.8V, and record the blank spectrum. Then add 2 ml of standard solutions with different concentrations to the supporting electrolyte, perform potential scanning and record the peak current value at -0.45V.

[0027] Correlate the peak currents produced by standard solutions with different vitamin K1 contents with the corresponding vitamin K1 contents t...

Embodiment 3

[0029] The repeatability of the electrode prepared by the method of the present invention for determining the content of vitamin K1 was investigated.

[0030] 10 titanium dioxide-modified glassy carbon electrodes were made by the method of Example 1, and the vitamin K1 current value of 1.0ng / ml was detected. The results are shown in Table 1, and the average value is 7.597±0.14μA (n=10, RSD=1.85%) , indicating that the titania-modified glassy carbon electrode prepared by this method has good preparation stability and repeatability.

[0031]

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Abstract

The invention provides a fast detecting method of a vitamin K1 used for preventing coupling hemorrhage after vaccine injection.The method comprises the specific operation steps of preparing electrodes, utilizing the electrodes to carry out testing, setting up a standard curve and preparing and measuring samples.The modified electrode and the unmodified glassy carbon electrode are adopted to carry out the electrochemical reaction of the vitamin K1, it is found through comparison that the current response of the modified electrode to the vitamin K1 is far larger than that of the unmodified glassy carbon electrode, it shows that the response of nanometer titanium oxide to the vitamin K1 has a certain sensibilization effect, due to the porous structure of the nanometer titanium oxide membrane electrode, the specific area is increased, accordingly, the adsorption characteristic is enhanced, and more vitamin K1 can be enriched.According to the method of using the modified electrode to detect the vitamin K1 in serum, the serum does not need to be subjected to complex pretreatment, it only takes 100 s to detect each sample, detecting is convenient and fast, and the requirement for detecting a large number of the serum samples can be fully met.

Description

technical field [0001] The invention relates to the field of electrochemical analysis, in particular to a rapid detection method for vitamin K1 used to prevent coupled bleeding after vaccine injection. Background technique [0002] Statistics show that the incidence of vitamin K deficiency in newborns is 4 / 10,000 to 10 / 10,000 live births, mostly in rural areas, and intracranial hemorrhage is the most common, accounting for about 60% to 80%. Late-onset vitamin deficiency is more common in newborns aged 2 weeks to 3 months, especially in breastfed infants aged about 1 month. On the other hand, one month after the newborn is born, it happens to be the second dose of hepatitis B vaccine. If a baby with vitamin K deficiency is inoculated with hepatitis B vaccine, bleeding at the injection site will often occur, and eventually intracranial hemorrhage may occur. infant death, related Coupling late-onset vitamin K deficiency in neonates after vaccination with hepatitis B vaccine ...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/48G01N27/308
Inventor 王城玉
Owner ZHONGYI ANKE BIOTECH CO LTD
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