Tamarind fruit shell extract, preparing method and application thereof in reducing glycosylated hemoglobin level
A technology of extract and tamarind, which is applied in the field of plant extract and its preparation, can solve the problems of rising blood sugar, prevention and control of diabetic complications, etc., to improve glucose tolerance, improve the level of glycosylated hemoglobin, The effect of inhibiting activity
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Embodiment 1
[0019] Embodiment one: the preparation of tamarind shell ethanol extract
[0020] Accurately weigh 500 g of dried tamarind shells, crush them with a pulverizer, and reclaim the finely powdered tamarind shells, add 10 times the amount of 50v / v% hydrous ethanol, heat and reflux for extraction for 120 minutes, and filter the extract through gauze. Extract once again under the same conditions, then filter through gauze, and combine the two extracts and filtrates. The solvent is recovered from the filtrate to obtain the extract of the tamarind shell ethanol extract.
Embodiment 2
[0021] Embodiment two: the preparation of tamarind shell water extract
[0022] Accurately weigh 500g of dried tamarind shells, crush them with a grinder, and recover the finely powdered tamarind shells, add 5 times the amount of purified water, heat and reflux for extraction for 120 minutes, filter the extract through gauze, and use the same conditions for the filter cake Extract once, then filter through gauze, and combine the two extract filtrates. After the filtrate is concentrated, it is freeze-dried to obtain the extract of the tamarind shell water extract.
Embodiment 3
[0023] Embodiment three: the chemical composition analysis of tamarind shell extract
[0024] Get 240 mg of the ethanol extract extract of Tamarinosa made in Example 1, dissolve it in 5% methanol, and analyze its chemical composition by liquid chromatography-mass spectrometry (LC-MS), wherein the liquid chromatography column adopts Cosmosil 5C18-MS-II chromatographic column (4.6ID×250mm, purchased from Japan Half Well Company), the mobile phase is methanol-water (0.2% HCl). After analysis, it was found that the ethanol extract of tamarind shell contains a large amount of flavonoid active compounds, including quercetin, kaempferol, morin, apigenin, naringenin and luteolin.
[0025] Get the tamarind shell water extract extract prepared in Example 2, and analyze its chemical composition according to the above-mentioned method. After analysis, it was found that the water extract of tamarind shell also contains a large amount of flavonoid active compounds, including quercetin, kae...
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