Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method for synthesizing fluorescent polymer nanoparticles on basis of polyethyleneimine

A polyethyleneimine and fluorescent polymer technology, applied in the field of nanomaterials, can solve the problems of cumbersome synthesis process, poor environmental protection and high cost, and achieve the effects of excellent fluorescence performance, strong stability and good stability

Inactive Publication Date: 2016-07-27
QUFU NORMAL UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of cumbersome synthesis process, high cost and poor environmental protection of fluorescent materials existing in the prior art, the present invention provides a preparation method for synthesizing fluorescent polymer nanoparticles based on polyethyleneimine, which directly uses polyethyleneimine as One-step Synthesis of Fluorescent Polymer Nanoparticles by Acylation Reaction of Matrix and Ascorbic Acid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for synthesizing fluorescent polymer nanoparticles on basis of polyethyleneimine
  • Preparation method for synthesizing fluorescent polymer nanoparticles on basis of polyethyleneimine
  • Preparation method for synthesizing fluorescent polymer nanoparticles on basis of polyethyleneimine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1.1 Weigh 0.7046g of polyethyleneimine (molecular weight: 600) into a 4mL plastic centrifuge tube, add 2mL of ultrapure water into it, shake and mix evenly to prepare A solution;

[0034] 1.2 Weigh 0.3523g of ascorbic acid into a 4mL plastic centrifuge tube, add 2mL of ultrapure water into it, shake and mix evenly to prepare B solution;

[0035] 1.3 Take 100 μL of solution B, add 50 μL of solution A to it, and then add 350 μL of ultrapure water, shake and mix evenly to obtain a fluorescent polymer nanoparticle solution;

[0036] The particle size of the fluorescent polymer nanoparticles prepared in this example is 9.1 nm, and it takes 14 days to stabilize, and the stable period is at least 3 months.

Embodiment 2

[0038] 2.1 Weigh 0.7046g polyethyleneimine (molecular weight: 1300) into a 4mL plastic centrifuge tube, add 2mL ultrapure water to it, shake and mix evenly to prepare A solution;

[0039] 2.2 Weigh 0.3523g of ascorbic acid into a 4mL plastic centrifuge tube, add 2mL of ultrapure water into it, shake and mix evenly to prepare B solution;

[0040] 2.3 Take 100 μL of solution B, add 100 μL of solution A to it, and then add 300 μL of ultrapure water, shake and mix evenly to obtain a fluorescent polymer nanoparticle solution;

[0041] The particle size of the fluorescent polymer nanoparticles prepared in this example is 9.2 nm, and it takes 13 days to stabilize, and the stable period is at least 3 months.

Embodiment 3

[0043] 3.1 Weigh 0.7046g polyethyleneimine (molecular weight: 1800) into a 4mL plastic centrifuge tube, add 2mL ultrapure water into it, shake and mix evenly to prepare A solution;

[0044] 3.2 Weigh 0.3523g of ascorbic acid into a 4mL plastic centrifuge tube, add 2mL of ultrapure water into it, shake and mix evenly to prepare B solution;

[0045] 3.3 Take 100 μL of solution B, add 150 μL of solution A to it, and then add 250 μL of ultrapure water, shake and mix evenly to obtain the fluorescent polymer nanoparticle solution;

[0046] The particle size of the fluorescent polymer nanoparticles prepared in this example is 9.2 nm, and it takes 13 days to stabilize, and the stable period is at least 3 months.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Radiusaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method for synthesizing fluorescent polymer nanoparticles on the basis of polyethyleneimine. The preparation method is implemented in steps as follows: solutions are prepared from polyethyleneimine and ascorbic acid respectively, mixed and then subjected to an acylation reaction, and the fluorescent polymer nanoparticles are prepared through one-step synthesis. The provided preparation method of the fluorescent polymer nanoparticles is simple and easy to operate, and the fluorescent polymer nanoparticles have good water solubility, have high fluorescence in the range of 350-370 nm and excellent stability, contain a large quantity of active groups allowed to be modified, can be used for the field of microimaging, biological probes, recognition and the like and have excellent application prospect.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and in particular relates to a preparation method for synthesizing fluorescent polymer nanoparticles based on polyethyleneimine. Background technique [0002] Polyethyleneimine (PEI) is a water-soluble high-density cationic polymer, a colorless or pale yellow viscous liquid, hygroscopic, soluble in water and ethanol, and insoluble in benzene. It has good water solubility, high cationicity, high reactivity and good gene transfection. In the prior art, the fluorescent nanomaterials synthesized by polyethyleneimine have complex synthesis steps and often contain noble metals, heavy metals, etc., such as gold, silver, sulfur, cadmium, zinc, etc., so that the related synthesis of the prepared fluorescent nanomaterials The matrix is ​​expensive, poor in environmental protection, and poor in biocompatibility. The molecular structural formula of polyethyleneimine is as follows: [0003] [0004] T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08G73/04C09K11/00
CPCC08G73/0206C09K11/00C09K2211/14
Inventor 曲斐王盛儒刘延群尤进茂
Owner QUFU NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com