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Method for preparing water-soluble fluorescence carbon dots and application of fluorescence carbon dots in resisting bacteria and distinguishing bacteria

A fluorescent carbon dot and water-soluble technology, which is applied in the field of nanomaterials, can solve the problems of insufficient distinguishing effect and complicated operation, and achieve the effects of wide application, low cytotoxicity, and excellent specific antibacterial performance

Active Publication Date: 2016-07-27
SOUTHEAST UNIV
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  • Application Information

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Problems solved by technology

However, the traditional Gram staining method is complicated to operate and the distinguishing effect is not obvious enough. Therefore, it is urgent to invent a new method that can easily and effectively distinguish Gram-negative bacteria from positive bacteria.

Method used

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  • Method for preparing water-soluble fluorescence carbon dots and application of fluorescence carbon dots in resisting bacteria and distinguishing bacteria
  • Method for preparing water-soluble fluorescence carbon dots and application of fluorescence carbon dots in resisting bacteria and distinguishing bacteria
  • Method for preparing water-soluble fluorescence carbon dots and application of fluorescence carbon dots in resisting bacteria and distinguishing bacteria

Examples

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Effect test

Embodiment 1

[0033] Solvothermal method to prepare water-soluble fluorescent antibacterial carbon dots, see the principle and process Figure 1a , Figure 1b , The method mainly includes the following steps:

[0034] (1) Preparation of carbon dots: add quaternary ammonium silane reagent to glycerin, stir and mix well, and react at 260°C for 4 hours in a hydrothermal reactor to obtain antibacterial carbon dots;

[0035] (2) Carbon dot purification: centrifugation or filtration to remove the precipitate, and the supernatant is dialyzed in water with a dialysis bag with a molecular weight cutoff of 1000 to obtain a pure carbon dot aqueous solution. The particle size characterization results (transmission electron microscope TEM) see Figure 2a with Figure 2b . Wherein, the volume fraction of the glycerol is 66.7%.

Embodiment 2

[0037] The solvothermal method is used to prepare water-soluble fluorescent antibacterial carbon dots. This group of examples is similar to Example 1, except that the reaction is carried out at 240° C. for 6 hours in a hydrothermal reaction kettle, and the volume fraction of the glycerin is 91%.

Embodiment 3

[0039] The solvothermal method is used to prepare water-soluble fluorescent antibacterial carbon dots. This example is similar to Example 1, except that the reaction is carried out at 280° C. for 2 hours in a hydrothermal reaction kettle, and the volume fraction of the glycerin is 50%.

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Abstract

The invention discloses a method for preparing water-soluble fluorescence carbon dots and application of the fluorescence carbon dots in resisting bacteria and distinguishing bacteria. The method for preparing the fluorescence carbon dots mainly comprises the steps of preparing carbon dots and purifying the carbon dots. According to the method, a quaternary ammonium salinized silane reagent and glycerol serve as raw materials, and the carbon dots with the surfaces quaternary ammonium salinized are successfully prepared through one step with a solvothermal method or a microwave method. The carbon dots can effectively kill gram-positive bacteria and can selectively achieve multicolor fluorescence imaging on gram-positive bacteria, and thus the carbon dots can be used for distinguishing gram-positive bacteria and negative bacteria. Besides, the carbon dots also have the advantages of being good in dispersity in an aqueous solution, low in cytotoxicity and cost, easy and convenient to purify and the like.

Description

Technical field [0001] The invention relates to nano materials, in particular to a method for preparing fluorescent carbon dots with antibacterial and bacterial imaging properties by adopting a one-step method, and water-soluble fluorescent carbon dots can selectively kill gram-positive bacteria and distinguish gram-positive bacteria And the application of negative bacteria. Background technique [0002] In recent years, various carbon materials (such as carbon nanotubes, carbon dots, graphene, fullerene, etc.) have gradually emerged and have attracted widespread attention. Carbon materials are widely used in biological detection, catalysis, energy, electronic devices, and drug loading due to their good biocompatibility and the advantages of cheap and readily available raw materials. As a new type of zero-dimensional carbon material, fluorescent carbon dots are widely used in drug carriers, bioimaging, biochemical detection, etc. due to its simple preparation process, good bioco...

Claims

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Application Information

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IPC IPC(8): C09K11/65C01B31/02B82Y40/00B82Y20/00G01N21/64A01N59/00A01P1/00
CPCA01N59/00B82Y20/00B82Y40/00C01P2004/02C01P2004/04C01P2004/51C01P2004/64C09K11/65G01N21/6486
Inventor 吴富根杨婧婧高歌
Owner SOUTHEAST UNIV
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