Fluorescent nanometer probe for detecting hydrogen sulfide and preparation method and application of fluorescent nanometer probe

A fluorescent probe, hydrogen sulfide technology, applied in the field of analysis, can solve the problems of cost increase, waste of organic liquid phase, etc., and achieve the effect of simple preparation

Inactive Publication Date: 2016-07-27
CAPITAL NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above method has the pretreatment of samples and the w

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  • Fluorescent nanometer probe for detecting hydrogen sulfide and preparation method and application of fluorescent nanometer probe
  • Fluorescent nanometer probe for detecting hydrogen sulfide and preparation method and application of fluorescent nanometer probe
  • Fluorescent nanometer probe for detecting hydrogen sulfide and preparation method and application of fluorescent nanometer probe

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Example Embodiment

[0043] Example 1

[0044] The first step, preparation and characterization of precursors

[0045] (a) Preparation of carbon quantum dots: Place 1g of Tris powder in a 10mL beaker, heat it to 230°C with a heating mantle and keep it for 20 minutes to melt it. When the melt changes from colorless to deep red, take it out and place it for a second time. Dilute in water. Use a fluorescence spectrophotometer to detect the fluorescence intensity of the system composed of secondary water and Tris, and continue to add water until the fluorescence intensity of the system reaches the maximum value and stop adding the secondary water to obtain nitrogen-containing carbon quantum dots, that is, CQDs. The pH is 10.

[0046] Characterization of CQDs: Use transmission electron microscope to characterize CQDs, and the results are as follows figure 2 Shown in A. It can be seen from the figure that CQDs are uniformly distributed in the water phase and have uniform particle size. According to statis...

Example Embodiment

[0057] Example 2

[0058] Keep the steps in Example 1 unchanged, fix the volume ratio of the aqueous solution of CQDs and AgNPs at 2:1, and only control the pH of CQDs at 7 experimental values ​​between 1-14: 1, 4, 6, 7, 8 , 10, 14 in order to optimize the experimental conditions for detecting hydrogen sulfide, so that Na 2 S is completely converted to H 2 S.

[0059] Use a fluorescence spectrophotometer to test the emission spectra of each sample under 350nm excitation, and record the fluorescence intensity value at the maximum emission position at 440nm, and then plot it with the pH value, such as Figure 4 B shows the effect of pH on CQDs (a) and CQDs-AgNPs nanoprobes (b). Each point is the fluorescence emission spectrum intensity at 440nm; curve c is the addition of 100nM to each 1mL sample of curve b Na 2 After 100μL of S solution, the intensity of the fluorescence emission spectrum at 440nm.

[0060] It can be seen from the figure that Na is added to the CQDs-AgNPs nanoprobe. ...

Example Embodiment

[0065] Example 3

[0066] Keep the steps in Example 1 unchanged, only adjust the pH of the probe to 7 and control the volume ratio of CQDs to the aqueous solution of nano-silver to 9 experimental values ​​between 1000:1 and 2:5: 100: 1, 50:1, 20:1, 10:1, 5:1, 2:1, 5:4, 1:1, 2:3, 1:2, 2:5.

[0067] Use a fluorescence spectrophotometer to test the emission spectrum of each sample under excitation at 350nm, and record the fluorescence intensity value F at the maximum emission position at 440nm 1 , And compare it with the initial value of CQDs F a Subtract to get the fluorescence quenching difference ΔF=F a -F 1 , And then plot the ratio of ΔF to CQDs and AgNPs. Such as Figure 4 As shown in A, and analyzed to get the following conclusions:

[0068] When CQDs occupies a large proportion, the quenching effect is not obvious; when the proportion of AgNPs is greater than CQDs, the quenching effect becomes stable.

[0069] Therefore, the optimal ratio of nitrogen-containing carbon quantum do...

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Abstract

The invention discloses a fluorescent nanometer probe for detecting hydrogen sulfide and a preparation method and application of the fluorescent nanometer probe.The fluorescent nanometer probe is formed by simply mixing carbon quantum dots containing the nitrogen element with an aqueous solution containing nanometer silver.According to the fluorescent nanometer probe and the preparation method and application thereof, after the quantum dot-nanometer silver fluorescent probe is assembled, representation is conducted, experiment conditions for detecting hydrogen sulfide are optimized, then linear detection and interferent analysis are conducted on hydrogen sulfide, and finally the fluorescent nanometer probe can be applied to detection and analysis of hydrogen sulfide in an actual biological sample.When used for detecting hydrogen sulfide, the fluorescent nanometer probe is excellent in sensitivity and stability, the minimum detection limit reaches 91 pM, and the fluorescent nanometer probe can be used for detecting hydrogen sulfide in the actual sample.

Description

technical field [0001] The invention belongs to the field of analysis, and relates to a fluorescent nano-probe for detecting hydrogen sulfide, a preparation method and application thereof. Background technique [0002] Hydrogen sulfide is a toxic gas, but as a newly discovered endogenous gas signal molecule, it has important biological activities and is widely involved in various physiological and pathological processes of the body [KimuraH, NagaiY, UmemuraK, KimuraY.Antioxid. Redox. Sign., 2005, 7:795.]. Hydrogen sulfide not only has a therapeutic effect on multiple systemic ischemic diseases, but also has a close relationship with the two-way regulation of neuropathic and inflammatory pain. Therefore, it is necessary to develop a sensory analysis method with high selectivity and rapid and sensitive detection of hydrogen sulfide. Several detection methods that have been practically used at present 2 S methods include: electrochemical method, electroluminescence method, h...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6428
Inventor 林雨青毕新雨王超罗静轩韩伊婧
Owner CAPITAL NORMAL UNIVERSITY
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