Biochemical micro-reaction system, high-throughput sequencing database builder and application

Abstract

The invention provides a biochemical micro-reaction system which comprises a first oily liquid, a second oily liquid, a water-based gene sample and a reaction reagent, wherein the density of the first oily liquid is greater than that of the second oily liquid; the water-based gene sample and the reaction reagent are embedded between the first oily liquid and the second oily liquid to form a sandwich structure; the water-based gene sample and the reaction reagent are immiscible and do not react with the first oily liquid and the second oily liquid; and the first oily liquid and the second oily liquid are immiscible. In the biochemical micro-reaction system, each independent sample in a biochemical reaction process can be completely controlled and separated to avoid cross contamination, and a large quantity of consumables can be saved. The invention also provides a high-throughput sequencing database builder based on the biochemical micro-reaction system and an application.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a biochemical micro-reaction system, a high-throughput sequencing library building instrument and its application. Background technique [0002] At present, the mainstream of the DNA sequencer market is the second-generation high-throughput sequencing platform, mainly including Illumina2000 / 2500 / 4000, Roche's 454 sequencer, and ABI's SOLID sequencer. , all require the preparation of sequencing libraries. The process of library construction (library preparation) involves the addition of gene samples, the addition of buffer and enzyme reagent mixture, PCR (polymerase chain reaction), magnetic bead purification, etc. The process is complex and the liquid system used is huge, resulting in mixed samples. and high risk of sample contamination. [0003] In order to solve the above difficulties, there are two existing automatic library preparation solutions as follows: one is based on ...

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Problems solved by technology

[0004] In the existing library construction methods, in order to ensure a relatively high concentration of target samples, the usual sample volume range is 200 μL, and the reagent volume is greater than 200 μL. The relatively large reaction volume will lead to high reagent costs, and it also requires a large amount of one-time use. Consumables (such as gun tips, etc.)

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